A colloidal gold immunochromatography test strip based on a monoclonal antibody for the rapid detection of triadimefon and triadimenol in foods. Issue 1 (1st January 2020)

• Record Type: Journal Article
• Authors: Lin, Lu; Song, Shanshan; Wu, Xiaoling; Liu, Liqiang; Kuang, Hua
• Is Part Of: Food and agricultural immunology. Volume 31:Issue 1(2020)
• Page Start: 475
• Page End: 488
• Publication Date: 2020-01-01
• Subjects: Triadimefon -- triadimenol -- monoclonal antibody -- ic-ELISA -- colloidal gold immunochromatography test strip; Immunochemistry -- Periodicals; Food -- Analysis -- Periodicals; Immunoassay -- Periodicals; 616.079
• DOI: 10.1080/09540105.2020.1736010
• Languages: English
• ISSNs: 0954-0105

Abstract

ABSTRACT: In this study, we produced a specific monoclonal antibody (mAb) against triadimefon (TDF) and triadimenol (TDN). The half-maximal inhibitory concentration for TDF and TDN was 5.25 and 9.98 ng/mL, respectively, with a linear detection range (IC20 –IC80 ) of 1.09–25.35 and 2.29–43.61 ng/mL, respectively. Subsequently, we developed a colloidal gold immunochromatography test strip for the analysis of TDF and TDN in tomato. The results obtained from the colloidal gold immunochromatography test strip were in accordance with those obtained from the indirect competitive enzyme-linked immunosorbent assay. The colloidal gold immunochromatography test strip is efficient and suitable for on-site detection of TDF and TDN.