A new model of the mechanism underlying lead poisoning: SNPs in miRNA target region influence the δ-aminolevulinic acid dehydratase expression level. (November 2017)

• Record Type: Journal Article
• Authors: Li, Chunping; Wang, Miaomiao; Wang, Yiqing; Zhang, Jinlong; Sun, Na
• Is Part Of: Epigenomics. Volume 9:Number 11(2017)
• Page Start: 1353
• Page End: 1361
• Publication Date: 2017-11
• Subjects: 3′-UTR -- ALAD -- lead poisoning -- miRNA -- SNPs; Epigenesis -- Periodicals; Genomics -- Periodicals; Chromatin -- Periodicals; DNA -- Periodicals; 572.87
• DOI: 10.2217/epi-2016-0180
• Languages: English
• ISSNs: 1750-1911

Abstract

Aim: To determine if SNPs located within the 3′-UTR of δ-aminolevulinic acid dehydratase (ALAD) can alter the risk of lead poisoning and the ALAD gene expression.Materials & methods: A case–control study was carried out to find the SNPs in miRNA target region. Luciferase reporter gene assay, qRT-PCR and Western blot was used to determine the relationship between miRNA and SNPs.Results: We found a significant association between rs818708 and the risk of lead poisoning. miR-545–5p was influenced by rs818708 variant and might result in a significant change in ALAD expression.Conclusion: rs818708 T > C can weaken the binding capability between miR-545–5p and 3′-UTR of ALAD and thus may alter the risk of lead poisoning.