Genome-wide integrative analysis identified SNP-miRNA-mRNA interaction networks in peripheral blood mononuclear cells. (October 2017)

• Record Type: Journal Article
• Authors: Lin, Xiang; Xia, Wei; Ji, Wei; Xie, Fang-Fei; He, Pei; Zhu, Xiao-Wei; Zhang, Yong-Hong; Deng, Fei-Yan; Lei, Shu-Feng
• Is Part Of: Epigenomics. Volume 9:Number 10(2017)
• Page Start: 1287
• Page End: 1298
• Publication Date: 2017-10
• Subjects: CIT -- eQTLs -- interaction networks -- miRNAs; Epigenesis -- Periodicals; Genomics -- Periodicals; Chromatin -- Periodicals; DNA -- Periodicals; 572.87
• DOI: 10.2217/epi-2017-0042
• Languages: English
• ISSNs: 1750-1911

Abstract

Aim: To detect SNP-miRNA-mRNA interaction networks and to elucidate miRNA-mediated regulation effects on mRNA expression.Materials & methods: In human peripheral blood mononuclear cells of 43 females, SNP-miRNA-mRNA interaction networks were established through an integrative analysis. Then causal inference test was followed to detect miRNA-mediated effects on mRNA expressions.Results: About 167 trios corresponding to 56 SNPs, 20 miRNAs and 47 target-mRNAs have the SNP-miRNA-mRNA interactions, but only 22 trios have miRNA-mediated effects between SNP and mRNA. For the three miRNAs (hsa-miR-222–3p, hsa-miR-181b-5p and hsa-miR-106b-5p), each mediates at least four correlations between SNP and mRNA. The mRNAs in interaction play an important role in energy metabolism, cellular and tissue homeostasis.Conclusion: This study represents the first effort of constructing an integrative interaction network of SNP-miRNA-mRNA and miRNA-mediated regulatory effects that provide helpful clues for future investigations of peripheral blood mononuclear cell-related physiological process and immunological diseases.