DNA methylation of imprinted genes in Mexican–American newborn children with prenatal phthalate exposure. (29th June 2018)

• Record Type: Journal Article
• Authors: Tindula, Gwen; Murphy, Susan K; Grenier, Carole; Huang, Zhiqing; Huen, Karen; Escudero-Fung, Maria; Bradman, Asa; Eskenazi, Brenda; Hoyo, Cathrine; Holland, Nina
• Is Part Of: Epigenomics. Volume 10:Number 7(2018)
• Page Start: 1011
• Page End: 1026
• Publication Date: 2018-06-29
• Subjects: cord blood -- differentially methylated region -- DNA methylation -- endocrine disruptors -- imprinted genes -- in utero exposure -- Mexican–Americans -- newborns -- phthalates -- pyrosequencing; Epigenesis -- Periodicals; Genomics -- Periodicals; Chromatin -- Periodicals; DNA -- Periodicals; 572.87
• DOI: 10.2217/epi-2017-0178
• Languages: English
• ISSNs: 1750-1911

Abstract

Aim: Imprinted genes exhibit expression in a parent-of-origin-dependent manner and are critical for child development. Recent limited evidence suggests that prenatal exposure to phthalates, ubiquitous endocrine disruptors, can affect their epigenetic dysregulation.Materials & methods: We quantified DNA methylation of nine imprinted gene differentially methylated regions by pyrosequencing in 296 cord blood DNA samples in a Mexican–American cohort. Fetal exposure was estimated by phthalate metabolite concentrations in maternal urine samples during pregnancy.Results: Several differentially methylated regions of imprinted genes were associated with high molecular weight phthalates. The most consistent, positive, and false discovery rate significant associations were observed for MEG3 .Conclusion: Phthalate exposure in utero may affect methylation status of imprinted genes in newborn children.