Vitamin B12 supplementation influences methylation of genes associated with Type 2 diabetes and its intermediate traits. (January 2018)

• Record Type: Journal Article
• Authors: Yadav, Dilip K; Shrestha, Smeeta; Lillycrop, Karen A; Joglekar, Charu V; Pan, Hong; Holbrook, Joanna D; Fall, Caroline HD; Yajnik, Chittaranjan S; Chandak, Giriraj R
• Is Part Of: Epigenomics. Volume 10:Number 1(2018)
• Page Start: 71
• Page End: 90
• Publication Date: 2018-01
• Subjects: DNA methylation -- folic acid -- miRNAs -- molecular mechanisms -- supplementation -- Type 2 diabetes -- vitamin B12; Epigenesis -- Periodicals; Genomics -- Periodicals; Chromatin -- Periodicals; DNA -- Periodicals; 572.87
• DOI: 10.2217/epi-2017-0102
• Languages: English
• ISSNs: 1750-1911

Abstract

Aim: To investigate the effect of B12 and/or folic acid supplementation on genome-wide DNA methylation.Methods: We performed Infinium HumanMethylation450 BeadChip (Zymo Research, CA, USA) assay in children supplemented with B12 and/or folic acid (n = 12 in each group) and investigated the functional mechanism of selected differentially methylated loci.Results: We noted significant methylation changes postsupplementation in B12 (589 differentially methylated CpGs and 2892 regions) and B12 + folic acid (169 differentially methylated CpGs and 3241 regions) groups. Type 2 diabetes-associated genes TCF7L2 and FTO; and a miRNA, miR21 were further investigated in another B12 -supplementation cohort. We also demonstrate that methylation influences miR21 expression and FTO, TCF7L2, CREBBP/CBP and SIRT1 are direct targets of miR21-3p .Conclusion: B12 supplementation influences regulation of several metabolically important Type 2 diabetes-associated genes through methylation of miR21 . Hence, our study provides novel epigenetic explanation for the association between disordered one carbon metabolism and risk of adiposity, insulin resistance and diabetes and has translational potential.