A cell retrievable strategy for harvesting extracellular matrix as active biointerface. (10th December 2022)

• Record Type: Journal Article
• Authors: Dong, Xiangyu; Zhang, Shuxiang; Xu, Yi; Chen, Longquan; Wei, Qiang; Zhao, Changsheng
• Is Part Of: Journal of materials science & technology. Volume 130(2022)
• Page Start: 44
• Page End: 52
• Publication Date: 2022-12-10
• Subjects: Extracellular matrix -- Regenerative medicine -- Biocoatings -- Cell sheets -- Differentiation; Metals -- Periodicals; Materials science -- Periodicals; Materials science; Metals; Periodicals; 620.1105
• DOI: 10.1016/j.jmst.2022.04.033
• Languages: English
• ISSNs: 1005-0302

Abstract

Highlights: An efficient approach is proposed to prepare ECM coatings without damaging cells. The detached cells are still active and can be reused to generate new ECM coatings. The obtained ECM coatings maintain the features of the natural ECM. The ECM coatings promote cell adhesion and guide the stem cell differentiation. This method can extract pure ECM coatings without cell residual and fragments. Abstract: Extracellular matrix (ECM) provides a variety of physical and chemical cues for cells. Here, a very simple and smart method is developed to glue living cells away for harvesting their ECMs. The obtained ECM coatings show less cell fragment residues comparing with those obtained by the traditional cell lysis. The glued cell sheets can even be re-cultured and reused after transferring to new environment. This moderate way well maintains the activity of the ECM proteins, which can promote cell adhesion and growth. Strikingly, the ECM coatings acquired from different functional cells can guide stem cell differentiation, which is attributed to the natural physical and biochemical cues on ECM coatings. Consequently, this method provides a substantial progress for preparing natural ECM coatings and shows promising potential in regenerative medicine and other related fields of biomedical engineering. Graphical abstract: Image, graphical abstract