Triterpenic and phenolic acids production changed in Salvia officinalis via in vitro and in vivo polyploidization: A consequence of altered genes expression. (September 2021)

• Record Type: Journal Article
• Authors: Tavan, Mansoureh; Sarikhani, Hassan; Mirjalili, Mohammad Hossein; Rigano, Maria Manuela; Azizi, Ali
• Is Part Of: Phytochemistry. Volume 189(2021)
• Publication Date: 2021-09
• Subjects: Salvia officinalis -- Lamiaceae -- Induced polyploidy -- Triterpenic acids -- Phenolic acids -- Gene expression; TAs triterpenic acids -- UA ursolic acid -- BA betulinic acid -- OA oleanolic acid -- RA rosmarinic acid -- SAA salvianolic acid A -- CA caffeic acid -- ChA chlorogenic acid -- FDS farnesyl pyrophosphate synthase -- SQS squalene synthase -- SQE squalene epoxidase -- LUS lupeol synthase -- MFAS mixed-function amyrin synthase -- BAS β-amyrin synthase -- PGRs plant growth regulators -- MS Murashige and Skoog -- HPLC high performance liquid chromatography -- FCM flow cytometer; Botanical chemistry -- Periodicals; Biochemistry -- Periodicals; Botany -- Periodicals; Chimie végétale -- Périodiques; 572.2
• DOI: 10.1016/j.phytochem.2021.112803
• Languages: English
• ISSNs: 0031-9422

Abstract

Abstract: The induction of polyploidy is an efficient technique for creating a diversity of genetic, phenotypic, and phytochemical novelties in plant taxa. Sage ( Salvia officinalis L.) is a well-known medicinal plant rich of valuable bioactive molecules such as triterpenic and phenolic acids. In the present study, the effect of in vitro and in vivo polyploidization on morphological characteristics, anatomical structures, phytochemical traits, and expression level of the genes involved in the biosynthesis of major triterpenic acids (ursolic, betulinic, and oleanolic acids) of the plant was studied. The sterile seeds treated with different concentrations (0, 0.05, 0.1, and 0.2%) of colchicine for 24 and 48 h were considered for polyploidy induction. Flow cytometry and chromosome counting were used to confirm the ploidy level of diploid (2 n = 2 x = 14, 2C DNA = 1.10 pg) and tetraploid (2 n = 4 x = 28, 2C DNA = 2.12 pg) plants after seven months. The highest polyploidy induction was obtained by applying 0.1% (w/v) colchicine for 48 h with an efficiency of 19.05% in vitro tetraploidy. Polyploids showed differences in leaf shape and color, leaf and stem thickness, trichrome density, root length, plant height, and number of leaves compared to diploid plants. There was also a significant decrease in rosmarinic acid content in polyploid (plants) as compared to diploid plants. Although a significant decrease in ursolic acid content was observed in polyploids, betulinic acid content associated with the expression levels of genes encoding enzymes being active in triterpene biosynthesis such as squalene epoxidase ( SQE ) and lupeol synthase ( LUS ). The expression of SQE and LUS was significantly increased in in vitro tertaploids (2.9-fold) and in vivo mixoploids (2.4-fold). The results confirm the idea that induced polyploidy can randomly alter breeding traits of plants as well as the content of bioactive compounds. Graphical abstract: Image 1 Highlights: Induction of polyploidy of both in vitro and in vivo conditions of S. officinalis was studied. The betulinic acid ‎content was significantly increased in in vitro tetraploids and ‎ in vivo mixoploids. The significant decrease in ‎ursolic acid and rosmarinic acid contents was observed in polyploid plants. The expression level of ‎lupeol synthase ( LUS )‎ gene was significantly increased in polyploid plants.