Glucose absorption regulation and mechanism of the compounds in Lilium lancifolium Thunb on Caco-2 cells. (March 2021)

• Record Type: Journal Article
• Authors: Xu, Xiaoqing; Wang, Pengyu; Wang, Baoguang; Wang, Mengke; Wang, Senye; Liu, Zhenhua; Zhang, Yan; Kang, Wenyi
• Is Part Of: Food and chemical toxicology. Volume 149(2021)
• Publication Date: 2021-03
• Subjects: Caco-2 cells -- SGLT1 -- GLUT2 -- Glucose uptake -- Kaempferol -- Caffeic acid -- Quercetin-3-O-β-D-galactoside; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2021.112010
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: In this paper, the Caco-2 cell was used to study the glucose absorption regulation and mechanism of kaempferol, caffeic acid and quercetin-3-O- β -D-galactoside in Lilium lancifolium Thunb in vitro . Glucose oxidase-peroxidase (GOD-POD) method was used to measure glucose consumption in supernatant. The fluorescent D-glucose analog (2-NBDG) was used as a tracer probe to study the changes in the fluorescence intensity of 2-NBDG uptake by Caco-2 cells with an inverted fluorescence microscope. Western blotting and quantitative real-time PCR were used to detect the protein expression and mRNA transcription of SGLT1 and GLUT2. The results showed that caffeic acid and quercetin-3-O- β -D-galactoside could significantly promote the absorption of glucose by normal Caco-2 cells compared with the control group ( P < 0.001). Both caffeic acid and quercetin-3-O- β -D-galactoside could significantly promote the uptake of glucose tracer 2-NBDG on Caco-2 cells. Caffeic acid and quercetin-3-O- β -D-galactoside could significantly promote SGLT1 and GLUT2 protein expression levels and mRNA transcription ( P < 0.001, P < 0.01, P < 0.05). The mechanism might be related to the promotion of SGLT1 and GLUT2 protein expression levels and mRNA transcription. Highlights: Caffeic acid and quercetin-3-O- β -D-galactoside could promote Caco-2 cell proliferation and glucose absorption. Caffeic acid and quercetin-3-O- β -D-galactoside could promote mRNA transcription levels of SGLT1 and GLUT2. Caffeic acid and quercetin-3-O-β-D-galactoside could up-regulation of SGLT1 and GLUT2 protein expression levels.