Solid-state bioprocessing of sugarcane bagasse with Auricularia fuscosuccinea for phenolic compounds extraction. Issue 6 (1st July 2022)

• Record Type: Journal Article
• Authors: Coniglio, Romina; Díaz, Gabriela; López, Cinthya; Restelli, María; Grassi, Emanuel; Albertó, Edgardo; Zapata, Pedro
• Is Part Of: Preparative biochemistry & biotechnology. Volume 52:Issue 6(2022)
• Page Start: 701
• Page End: 710
• Publication Date: 2022-07-01
• Subjects: Auricularia fuscosuccinea -- β-glucosidases -- DPPH -- molecular identification -- solid state-bioprocessing -- sugarcane bagasse -- total phenolic content; Biochemistry -- Technique -- Periodicals; Biotechnology -- Technique -- Periodicals; Biochemistry -- methods -- Periodicals; Biotechnology -- methods -- Periodicals; 660.6
• DOI: 10.1080/10826068.2021.1986722
• Languages: English
• ISSNs: 1082-6068

Abstract

Abstract: Sugarcane bagasse is a natural source of phenolic compounds. However, these compounds are bound to lignocellulose components, reducing their ability to function as good antioxidants. These linkages are hydrolyzed by enzymes like β -glucosidases, increasing free phenolics. Auricularia is a food-grade genus capable of producing β -glucosidases. The aim of this work was (I) to determine naturally occurring species of Auricularia and (II) to obtain phenolic compounds through the solid-state bioprocessing of sugarcane bagasse. We have successfully isolated five strains that were assigned to the taxon A. fuscosuccinea . We determined β -glucosidase activity by fluorescence plate assay of the five isolated strains and adjusted an optimal temperature for mycelial growth at 30 °C. A. fuscosuccinea LBM 243 was chosen for solid-state bioprocessing of sugarcane bagasse. β -glucosidase activity (12.2 ± 0.62 U l −1 ) and protein content (51.58 ± 6.26 mg l −1 ) were highest on day 20 of culture. The maximum value of total phenolic content (507.5 ± 9.05 mg l −1 ) was obtained at day 20 and antioxidant capacity (34.44% ± 11.20) was highest at day 10, both in ethanolic extracts. The best performance of ethanol against methanol extraction in this work is highlighted considering ethanol to be a safe, efficient, and low-cost solvent.