Cyanogenic glycoside amygdalin influences functions of human osteoblasts in vitro. Issue 2 (1st February 2021)

• Record Type: Journal Article
• Authors: Omelka, Radoslav; Kovacova, Veronika; Mondockova, Vladimira; Grosskopf, Birgit; Kolesarova, Adriana; Martiniakova, Monika
• Is Part Of: Journal of environmental science and health. Volume 56:Issue 2(2021)
• Page Start: 109
• Page End: 116
• Publication Date: 2021-02-01
• Subjects: Amygdalin -- osteoblasts -- viability -- morphology -- gene expression; Pesticides -- Environmental aspects -- Periodicals; Food contamination -- Periodicals; Agricultural wastes -- Environmental aspects -- Periodicals; Ecology -- periodicals; Pesticides -- toxicity -- periodicals; Food Contamination -- periodicals; 363.73
• DOI: 10.1080/03601234.2020.1852054
• Languages: English
• ISSNs: 0360-1234

Abstract

Abstract: Amygdalin has been promoted as an alternative cancer cure. However, it is still unclear how this cyanogenic glycoside affects non-cancer cells including bone cells. This study first investigated the impact of amygdalin on viability, morphology and expression of important genes in human osteoblasts in vitro . Primary human osteoblast cultures were exposed to amygdalin at concentrations 0; 0.1; 1 and 10 mg/mL in growth medium for 72 h. Cell viability, osteoblasts morphology and expression of 10 genes associated with osteoblast-specific pathways, oxidative stress and cell death were determined. Osteoblasts viability was significantly decreased (–27.26%) and their size was reduced (–23.20%) at the highest concentration of amygdalin (10 mg/mL). This concentration of amygdalin down-regulated the expression of COL1A1 and ALPL genes, whereas the expression of BGLAP, TNFSF11 and WNT5A genes was increased. The osteoblast cultivation with 0.1 mg/mL amygdalin caused down-regulation of COL1A1 gene. No changes in expression were determined for RUNX2, BAX, CASP1, SOD1 and GPX1 genes among all tested concentrations of amygdalin. In conclusion, amygdalin in a high concentration negatively affected mineralization of extracellular matrix, increased bone resorption and decreased osteoblast viability. These changes were accompanied by modified expression profiles of responsible genes.