Broad-specific monoclonal antibody based IACs purification coupled UPLC-MS/MS method for T-2 and HT-2 toxin determination in maize and cherry samples. Issue 1 (1st January 2020)

• Record Type: Journal Article
• Authors: Li, Yanshen; Lin, Shaoxia; Wang, Yunhui; Mao, Xin; Wu, Yongning; Liu, Yunguo; Chen, Daquan
• Is Part Of: Food and agricultural immunology. Volume 31:Issue 1(2020)
• Page Start: 291
• Page End: 302
• Publication Date: 2020-01-01
• Subjects: Broad-specific immune-affinity columns -- T-2 toxin -- HT-2 toxin -- UPLC-MS/MS; Immunochemistry -- Periodicals; Food -- Analysis -- Periodicals; Immunoassay -- Periodicals; 616.079
• DOI: 10.1080/09540105.2020.1724895
• Languages: English
• ISSNs: 0954-0105

Abstract

ABSTRACT: In this work, broad-specific monoclonal antibody based immunoaffinity columns (IACs) coupled to ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) for purification and determination of T-2 and HT-2 toxin in maize and cherry samples. Based on broad-specific anti-T-2 monoclonal antibody, the prepared IACs could be used for both T-2 and HT-2 purification with cross reactivity values at 100% for T-2 and 108% for HT-2. Column capacity was 3.14 μg mL −1 gel for T-2 toxin, and 3.20 μg mL −1 gel for HT-2 toxin. After 10 cycles of usage at intervals of 2 days, column capacity decreased to 0.85 μg mL −1 gel for T-2 and 1.22 μg mL −1 gel for HT-2 toxin. The developed method exhibited high sensitive results with limit of detection (LOD) lower than 0.35 μg kg −1 and 1.42 μg kg −1 .