Anti-idiotypic VHH mediated environmentally friendly immunoassay for citrinin without mycotoxin. Issue 1 (1st January 2020)

• Record Type: Journal Article
• Authors: Xiong, Liang; Zhang, Xueqin; Xu, Yang; Li, Yanping; Liu, Deguang; Tu, Zhui; He, Qinghua
• Is Part Of: Food and agricultural immunology. Volume 31:Issue 1(2020)
• Page Start: 968
• Page End: 984
• Publication Date: 2020-01-01
• Subjects: Anti-idiotypic single domain heavy chain antibody -- citrinin -- hapten mimicry -- environmentally friendly immunoassay; Immunochemistry -- Periodicals; Food -- Analysis -- Periodicals; Immunoassay -- Periodicals; 616.079
• DOI: 10.1080/09540105.2020.1795631
• Languages: English
• ISSNs: 0954-0105

Abstract

ABSTRACT: Competitive enzyme-linked immunosorbent assay (ELISA) is the most widely used method for rapidly screening of mycotoxins in contaminated food products; however, it involves the use of toxin-protein conjugates as a competitive antigen and toxin standards as calibrators. In this work, a single domain heavy chain antibody (VHH)-based environmentally friendly ELISA (VHH-ELISA) was developed to measure citrinin concentration in Monascus-fermented rice. With this novel indirect competitive VHH-ELISA, the use of a synthetic antigen and pure toxins for operation and environmental safety simultaneously avoided. The assay exhibited a limit of detection of 2.25 ng/mL, with a linear range of 5.0−500.0 ng/mL. VHH-ELISA was then utilized for sample testing, and excellent agreement was observed between VHH-ELISA and high-performance liquid chromatography as a reference. This study provided a novel platform to develop environmentally friendly immunoassays for other toxic small molecules in the application of anti-idiotypic VHHs.