Verification of Low-Density Lipoprotein Cholesterol Levels Measured by Anion-Exchange High-Performance Liquid Chromatography in Comparison with Beta Quantification Reference Measurement Procedure. (4th November 2020)

• Record Type: Journal Article
• Authors: Manita, Daisuke; Yoshida, Hiroshi; Koyama, Isao; Nakamura, Masakazu; Hirowatari, Yuji
• Is Part Of: Journal of applied laboratory medicine. Volume 6:Number 3(2021)
• Page Start: 654
• Page End: 667
• Publication Date: 2020-11-04
• Subjects: anion-exchange HPLC -- beta quantification -- homogeneous assay -- hypertriglyceridemia -- Sampson equation; Clinical chemistry -- Periodicals; Diagnosis, Laboratory -- Periodicals; 616.0756
• DOI: 10.1093/jalm/jfaa144
• Languages: English
• ISSNs: 2576-9456

Abstract

Abstract: Background: A new lipoprotein testing method based on anion-exchange HPLC (AEX-HPLC) was recently established. We verified the accuracy of LDL-C levels, a primary therapeutic target for the prevention of cardiovascular disease (CVD), measured by AEX-HPLC comparing with LDL-C levels measured by beta quantification-reference measurement procedure (BQ-RMP), homogenous assays, and calculation methods. Methods: We compared LDL-C levels measured by AEX-HPLC (adLDL-Ch: LDL-Ch and IDL-Ch) and BQ-RMP using blood samples from 52 volunteers. AdLDL-Ch levels were also compared with those measurements by homogeneous assays and calculation methods (Friedewald equation, Martin equation, and Sampson equation) using blood samples from 411 participants with dyslipidemia and/or type 2 diabetes. Results: The precision and accuracy of adLDL-Ch were verified by BQ-RMP. The mean percentage bias [bias (%)] for LDL-C was 1.2%, and the correlation was y = 0.990 x + 3.361 ( r = 0.990). These results met the acceptable range of accuracy prescribed by the National Cholesterol Education Program. Additionally, adLDL-Ch levels were correlated with LDL-C levels measured by the 2 homogeneous assays ( r > 0.967) and the calculation methods ( r > 0.939), in serum samples from patients with hypertriglyceridemia. Conclusions: AEX-HPLC is a reliable method for measuring LDL-C levels for CVD risk in daily clinical laboratory analyses.