Arthrobacter terricola sp. nov., isolated from forest soil. Issue 3 (8th March 2021)

• Record Type: Journal Article
• Authors: Trinh, Ngoc Hoang; Kim, Jaisoo
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 3(2021)
• Publication Date: 2021-03-08
• Subjects: Actinobacteria -- Arthrobacter terricola -- forest soil -- novel species -- Suwon; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004686
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-positive, aerobic and rod-shaped bacterial strain, designated JH1-1 T, was isolated from a forest soil sample collected in Suwon, Gyeonggi-do, Republic of Korea. Strain JH1-1 T could grow at 10–35 °C (optimum, 28–30 °C), pH 4.5–8.5 and tolerated 5 % (w/v) NaCl. Strain JH1-1 T was most closely related to members of the genus Arthrobacter, namely Arthrobacter alkaliphilus LC6 T (98.5 % similarity), Arthrobacter methylotrophus TGA T (98.4 %), Arthrobacter ramosus CCM 1646 T (97.8 %), Arthrobacter bambusae THG-GM18 T (97.5 %) and Arthrobacter pokkalii P3B162 T (97.3 %). The strain grew well on Reasoner's 2A agar, tryptone soya agar, nutrient agar, Mueller–Hinton agar and Luria–Bertani agar. The major polar lipid profile comprised phosphatidylglycerol, diphosphatidylglycerol, unidentified phospholipid and unidentified glycolipids. The major respiratory quinone was MK-9(H2 ). The main fatty acids were C15 : 0 anteiso, C15 : 0 iso, C16 : 0 iso and C17 :0 anteiso. The DNA G+C content of the isolated strain based on the whole genome sequence was 63.6 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain JH1-1 T and its reference type strains ranged from 81.3 to 85.4 % and from 21.1 to 29.1 %, respectively. Based on phenotypic, chemotypic and genotypic evidence, strain JH1-1 T could be differentiated phylogenetically and phenotypically from the recognized species of the genus Arthrobacter . Therefore, strain JH1-1 T is considered to represent a novel species, for which the name Arthrobacter terricola sp. nov. is proposed. The type strain is JH1-1 T (=KACC 21385 T =JCM 33641 T ).