Wnt signalling inhibits adipogenesis in orbital fibroblasts from patients with Graves' orbitopathy. Issue 7 (30th June 2021)

• Record Type: Journal Article
• Authors: Jung, Sang Joon; Choi, Yeon Jeong; Park, Tae Kwann; Woo, Sang Earn; Kim, Bo-Yeon; Yoon, Jin Sook; Jang, Sun Young
• Is Part Of: British journal of ophthalmology. Volume 106:Issue 7(2022)
• Page Start: 1019
• Page End: 1027
• Publication Date: 2021-06-30
• Subjects: orbit -- experimental and laboratory; Ophthalmology -- Periodicals; 617.7
• DOI: 10.1136/bjophthalmol-2020-316898
• Languages: English
• ISSNs: 0007-1161

Abstract

Abstract : Background/Aims: To investigate the role of Wnt signalling in adipogenesis using an in vitro model of Graves' orbitopathy (GO). Methods: Orbital fat was obtained from patients with GO and non-GO participants for primary orbital fibroblast (OF) culture. Expression levels of Wnt5a, Wnt10b, β-catenin, phospho-β-catenin and cyclin D1 were compared between GO and non-GO OFs. These expression levels were also determined during adipogenesis of GO and non-GO OFs. The effects of a stimulator and inhibitor of Wnt signalling on adipogenesis of GO and non-GO OFs were investigated. Results: Western blotting analysis showed significant reductions in β-catenin and cyclin D1 and significant enhancement of phospho-β-catenin in OFs from patients with GO, compared with OFs from non-GO participants (p<0.05). Expression of Wnt5a, Wnt10b, β-catenin and cyclin D1 in OFs was highest on day 0, and then gradually declined after induction of adipogenic differentiation. The expression levels of PPARγ, C/EBPα and C/EBPβ were reduced in Wnt stimulator-treated OFs in a dose-dependent manner. Oil red O staining confirmed that a stimulator of Wnt inhibited adipogenesis in GO OFs. Conclusion: These results indicate that Wnt signalling inhibits adipogenesis in OFs from patients with GO and non-GO participants. Further studies are required to examine the potential of Wnt signalling as a target for therapeutic strategies.