Raising the Bar(-seq) in Leishmania Genetic Screens. Issue 5 (May 2021)

• Record Type: Journal Article
• Authors: Ferreira, Tiago R.; Couñago, Rafael M.; Moretti, Nilmar S.
• Is Part Of: Trends in parasitology. Volume 37:Issue 5(2021)
• Page Start: 367
• Page End: 369
• Publication Date: 2021-05
• Subjects: bar-seq -- NGS -- Leishmania -- Cas9 -- kinome -- kinase; Parasitology -- Periodicals; Parasitology -- Periodicals; Biology -- Periodicals; Parasitology; Biology; Parasitologie -- Périodiques; Online resources; 571.999
• DOI: 10.1016/j.pt.2021.03.002
• Languages: English
• ISSNs: 1471-4922

Abstract

Abstract : Our understanding of regulatory factors in Leishmania differentiation has long been restricted by the available genetic tools, but the availability of CRISPR/Cas9 has changed the landscape forever. Recently, Baker and Catta-Preta et al. applied Cas9 editing and kinome-wide bar-seq to dissect the function of 204 kinases in the Leishmania mexicana life cycle.