CATCH: high specific transcriptome-focused fusion gene variants discrimination. Issue 55 (22nd June 2022)

• Record Type: Journal Article
• Authors: Yuan, Rui; Bai, Xiaopeng; Hu, Xiaolin; Zhang, Hong; Hou, Changjun; Long, Quanxin; Luo, Yang
• Is Part Of: Chemical communications. Volume 58:Issue 55(2022)
• Page Start: 7618
• Page End: 7621
• Publication Date: 2022-06-22
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d2cc02660k
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A CRISPR-Cas13a triggered catalytic hairpin assembly (CATCH) approach for accurate and impartial identification of fusion gene variants. Abstract : We develop a CRISPR-Cas13a triggered catalytic hairpin assembly (CATCH) approach for accurate and impartial identification of variants by integrating the fusion gene-selected recognition of CRISPR-Cas13a with collateral cleavage-assisted catalytic hairpin assembly amplification. This approach achieved an accuracy of 100% in a pilot experiment involving 34 clinical samples.