Degradation of Shanxi lignite by Trichoderma citrinoviride. (1st May 2021)
- Record Type:
- Journal Article
- Title:
- Degradation of Shanxi lignite by Trichoderma citrinoviride. (1st May 2021)
- Main Title:
- Degradation of Shanxi lignite by Trichoderma citrinoviride
- Authors:
- Feng, Xin
Sun, Jinghong
Xie, Yuhong - Abstract:
- Highlights: The A-1 strain solubilized 35% (by weight) of Shanxi lignite within 20 days. The A-1 fungal strain had the ability of dehydroxylate and demethylate. The extracellular enzyme is the key to the biodegradation mechanism of lignite. The degradation mechanism was the combination of multiple extracellular enzymes. Abstract: A fungal isolate A-1, identified as Trichoderma citrinoviride by sequencing of the 26S rDNA D1/D2 region, was isolated from the seeds of white Tremella. This fungus was used to degrade the Shanxi lignite. The intensity of the bio-solubilization was determined by measuring the weight loss of the lignite gravimetrically. This fungus solubilized 35% (by weight) of Shanxi lignite after H2 O2 pretreatment within 20 days. The enzyme activity analysis showed that the fungus could produce oxidase, lignin peroxidase, and laccase. The degradation mechanism of the fungus was the combined action of multiple extracellular enzymes, which achieved a high degradation efficiency. The lignite and the bio-solubilization products were characterized by ultraviolet spectroscopy (UV), infrared spectroscopy (FT-IR), gas chromatography-mass spectrometry (GC–MS), and X-ray diffraction (XRD). Results showed that the A-1 fungal strain had the abilities of dehydroxylation and demethylation, as well as breaking the side chains of the aromatic rings. After coal degradation, the solid residue mainly contains organic functional groups such as hydroxyl, aromatic ring, and esters,Highlights: The A-1 strain solubilized 35% (by weight) of Shanxi lignite within 20 days. The A-1 fungal strain had the ability of dehydroxylate and demethylate. The extracellular enzyme is the key to the biodegradation mechanism of lignite. The degradation mechanism was the combination of multiple extracellular enzymes. Abstract: A fungal isolate A-1, identified as Trichoderma citrinoviride by sequencing of the 26S rDNA D1/D2 region, was isolated from the seeds of white Tremella. This fungus was used to degrade the Shanxi lignite. The intensity of the bio-solubilization was determined by measuring the weight loss of the lignite gravimetrically. This fungus solubilized 35% (by weight) of Shanxi lignite after H2 O2 pretreatment within 20 days. The enzyme activity analysis showed that the fungus could produce oxidase, lignin peroxidase, and laccase. The degradation mechanism of the fungus was the combined action of multiple extracellular enzymes, which achieved a high degradation efficiency. The lignite and the bio-solubilization products were characterized by ultraviolet spectroscopy (UV), infrared spectroscopy (FT-IR), gas chromatography-mass spectrometry (GC–MS), and X-ray diffraction (XRD). Results showed that the A-1 fungal strain had the abilities of dehydroxylation and demethylation, as well as breaking the side chains of the aromatic rings. After coal degradation, the solid residue mainly contains organic functional groups such as hydroxyl, aromatic ring, and esters, and the liquid product contains aliphatic compounds such as alkanes, alcohols, esters, and phenolic organics. The main characteristic peaks of lignite were quartz (SiO2 ) and calcite (CaCO3 ). The relevant characteristic peaks of CaCO3 disappear after the coal sample was pretreated with H2 O2, indicating that the pretreatment process relaxed the interaction between coal macromolecules and improved the microbial degradation ability of coal. Trichoderma citrinoviride A-1 can promote biodegradation, and the extracellular enzyme contained in this fungus is the key to the biodegradation mechanism of lignite. … (more)
- Is Part Of:
- Fuel. Volume 291(2021)
- Journal:
- Fuel
- Issue:
- Volume 291(2021)
- Issue Display:
- Volume 291, Issue 2021 (2021)
- Year:
- 2021
- Volume:
- 291
- Issue:
- 2021
- Issue Sort Value:
- 2021-0291-2021-0000
- Page Start:
- Page End:
- Publication Date:
- 2021-05-01
- Subjects:
- Lignite -- Trichoderma citrinoviride -- Bio-solubilization -- GC–MS -- Enzyme activity
Fuel -- Periodicals
Coal -- Periodicals
Coal
Fuel
Periodicals
662.6 - Journal URLs:
- http://www.sciencedirect.com/science/journal/latest/00162361 ↗
http://www.elsevier.com/journals ↗ - DOI:
- 10.1016/j.fuel.2021.120204 ↗
- Languages:
- English
- ISSNs:
- 0016-2361
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 4048.000000
British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 22345.xml