Evaluating Sardinella brasiliensis quality indicators through the quantification of histamine and bacterial communities. Issue 8 (August 2020)

• Record Type: Journal Article
• Authors: de Lira, Alessandra Danile; de Castro, Icaro Maia Santos; Mann, Michele Bertoni; Mallmann, Luana Peixoto; Kothe, Caroline Isabel; Varela, Ana Paula Muterle; Frazzon, Ana Paula Guedes; Frazzon, Jeverson
• Is Part Of: Heliyon. Volume 6:Issue 8(2020)
• Publication Date: 2020-08
• Subjects: Food science -- Food analysis -- Food safety -- Microbiology -- Histamine -- Histidine decarboxylase -- High throughput sequencing (HTS) -- Microbiota -- qPCR -- HPLC-DAD; Research -- Periodicals; Medical sciences -- Periodicals; Natural history -- Periodicals; Social sciences -- Periodicals; Earth sciences -- Periodicals; Physical sciences -- Periodicals; 507.2
• DOI: 10.1016/j.heliyon.2020.e04461
• Languages: English
• ISSNs: 2405-8440

Abstract

Abstract: Primarily formed by the microbial decarboxylation of the amino acid histidine, histamine is the leading global cause of food poisoning from fish consumption worldwide. In the present work, the quality of 12 fresh and 12 frozen marketed sardines ( Sardinella brasiliensis ) were evaluated for histamine concentration using High-performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD), while the detection and quantification of histamine-producing bacteria were performed via quantitative Polymerase Chain Reaction (qPCR), and the microbiota composition of sardines was assessed through amplification of the 16S rRNA gene using high-throughput sequencing (HTS). According to the results obtained by HPLC-DAD, histamine concentration ranged from 226.14 to 583.87 mg kg −1 . The histidine decarboxylase ( hdc ) genes from gram-negative bacteria ( Morganella morganii, and Enterobacter aerogenes ) were identified. The most abundant microorganisms present in fresh sardines belong to the genera Macrococcus spp., Acinetobacter spp., and Pseudomonas spp., while the genera Phyllobacterium spp., Pseudomonas spp., and Acinetobacter spp. were most abundant in frozen sardines. Abstract : Food science; Food analysis; Food safety; Microbiology; Histamine; Histidine decarboxylase; High throughput sequencing (HTS); Microbiota; qPCR; HPLC-DAD