A novel polarity-sensitive fluorescent probe for lighting up lipid droplets and its application in discriminating dead and living zebrafish. (August 2022)

• Record Type: Journal Article
• Authors: Cui, Wei-Long; Wang, Mao-Hua; Chen, Xiao-Qian; Zhang, Zhi-Hao; Qu, Jianbo; Wang, Jian-Yong
• Is Part Of: Dyes and pigments. Volume 204(2022)
• Publication Date: 2022-08
• Subjects: Lipid droplets -- Polarity-sensitive -- Fluorescent probe -- Cell imaging -- Zebrafish; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2022.110433
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: In the present study, we developed a novel fluorescent chemosensor with a hybrid structure of 3-pyridinonitrile-coumarin derivatives by molecular design strategy that can effectively label intracellular lipid droplets. Interestingly, the probe ATOP-LD also exhibited polarity-sensitive properties, which may be attributable to its own strong electron-giving and electron-absorbing moieties. In addition, the probe exhibited excellent interference resistance, pH stability and low biotoxicity and had been successfully applied to cell imaging. Also, probe ATOP-LD could discriminate dead and living zebrafish or its embryos. These satisfactory properties prove that the probe is useful and possesses great potential for application. Abstract: Lipid droplets (LDs) represent essential intracellular organelles that store and supply lipids, which are associated with the synthesis of many disease-related intracellular signalling molecules. Aberrant expression of lipid droplets may cause cytotoxicity, cellular dysfunction and apoptosis. Similarly, as a very critical parameter and indicator of normal cells, polarity performs a vital function in many biological processes and physiological environments. In the present study, we developed a novel fluorescent chemosensor by molecular design strategy that can effectively label intracellular lipid droplets. Interestingly, the probe ATOP-LD also exhibited polarity-sensitive properties, which may be attributable to its own strong electron-giving and electron-absorbing moieties. Iinaten addition, the probe exhibited excellent interference resistance, pH stability and low biotoxicity, and had been successfully applied to cell imaging. Also, probe ATOP-LD could discriminate dead and living zebrafish or its embryos. Image 1 Highlights: A novel fluorescent chemosensor by molecular design strategy was developed, which can effectively label intracellular lipid droplets. The probe ATOP-LD also exhibited polarity-sensitive properties, which may be attributable to its own strong electron-giving and electron-absorbing moieties. The probe exhibited excellent interference resistance, pH stability and low biotoxicity. Probe ATOP-LD had been successfully applied to cell imaging, zebrafish and its embryos imaging. The probe was useful to discriminate dead and living zebrafish, which is a very cleaver application.