Assessing multimodal optical imaging of perfusion in burn wounds. Issue 4 (June 2022)

• Record Type: Journal Article
• Authors: Lertsakdadet, Ben S.; Kennedy, Gordon T.; Stone, Randolph; Kowalczewski, Christine; Kowalczewski, Andrew C.; Natesan, Shanmugasundaram; Christy, Robert J.; Durkin, Anthony J.; Choi, Bernard
• Is Part Of: Burns. Volume 48:Issue 4(2022)
• Page Start: 799
• Page End: 807
• Publication Date: 2022-06
• Subjects: A constant dependent on boundary conditions -- AAALAC Association for Assessment and Accreditation of Laboratory Animal Care -- CCD charge-coupled device -- DB Brownian diffusion coefficient -- FDA Food and Drug Administration -- FOV field of view -- G1 intensity autocorrelation function -- IACUC Institutional Animal Care and Use Committee -- K speckle contrast -- k0 wavenumber of light -- LSI laser speckle imaging -- n refractive index -- P0 incident optical power -- q laser source intensity -- ROI region of interest -- SFDI spatial frequency domain imaging -- SFI speckle flow index -- T exposure time -- USAISR United States Army Institute for Surgical Research -- mean intensity -- <Δr2(τ)> mean square displacement -- β factor accounting for polarization and speckle/pixel size ratio -- λ laser wavelength -- μa absorption coefficient -- μa, dyn dynamic optical property term -- μeff effective attenuation coefficient -- μsʹ reduced scattering coefficient -- μtr transport coefficient -- σ standard deviation of laser intensity; Burns -- Spatial frequency domain imaging -- Laser speckle contrast imaging -- Perfusion -- Blood flow -- Optical properties; Burns and scalds -- Periodicals; 617.11
• DOI: 10.1016/j.burns.2021.08.026
• Languages: English
• ISSNs: 0305-4179

Abstract

Highlights: Both optical properties and laser speckle-based measurements of blood flow change in response to burn injury. Optical property changes generally can affect the interpretation of laser speckle measurements as a blood-flow metric. Even with dynamic optical properties following burn injury, blood flow changes are well represented by laser speckle imaging. Our data suggest that laser speckle imaging is a reliable technology for assessing skin perfusion changes associated with burn wounds. Abstract: A critical need exists for early, accurate diagnosis of burn wound severity to help identify the course of treatment and outcome of the wound. Laser speckle imaging (LSI) is a promising blood perfusion imaging approach, but it does not account for changes in tissue optical properties that can occur with burn wounds, which are highly dynamic environments. Here, we studied optical property dynamics following burn injury and debridement and the associated impact on interpretation of LSI measurements of skin perfusion. We used spatial frequency domain imaging (SFDI) measurements of tissue optical properties to study the impact of burn-induced changes in these properties on LSI measurements. An established preclinical porcine model of burn injury was used (n = 8). SFDI and LSI data were collected from burn wounds of varying severity. SFDI measurements demonstrate that optical properties change in response to burn injury in a porcine model. We then apply theoretical modeling to demonstrate that the measured range of optical property changes can affect the interpretation of LSI measurements of blood flow, but this effect is minimal for most of the measured data. Collectively, our results indicate that, even with a dynamic burn wound environment, blood-flow measurements with LSI can serve as an appropriate strategy for accurate assessment of burn severity.