Fermentative production of 2, 3-Butanediol using bread waste – A green approach for sustainable management of food waste. (August 2022)

• Record Type: Journal Article
• Authors: Narisetty, Vivek; Zhang, Le; Zhang, Jingxin; Sze Ki Lin, Carol; Wah Tong, Yen; Loke Show, Pau; Kant Bhatia, Shashi; Misra, Ashish; Kumar, Vinod
• Is Part Of: Bioresource technology. Volume 358(2022)
• Publication Date: 2022-08
• Subjects: Bread waste -- Enterobacter ludwigii -- Dextrozyme Peak -- Hydrolysate -- 2, 3-Butanediol; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.127381
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Evaluation of bread waste (BW) as feedstock for 2, 3-butanediol (BDO) production. Glucose yield of 330–530 g/kg BW was obtained by acid and enzymatic hydrolysis. BDO titer of 144.5 g/L and yield of 0.47 g/g was achieved on pure glucose. BDO titer of 135.4 g/L with a yield of 0.42 g/g was amassed from acid hydrolysed BW. Enzyme hydrolysed BW generated 138.8 g/L BDO with a yield of 0.48 g/g. Abstract: Bread is Europe's most wasted food, and the second most wasted food after potatoes in UK. Bread waste (BW) is a clean source of high-quality fermentable sugars. In this study, the potential of Enterobacter ludwigii to accumulate 2, 3-butanediol (BDO) from BW was evaluated. Initially, the optimal inoculum size and yeast extract concentration were determined, followed by extraction of sugars from BW using acid and enzymatic hydrolysis. A glucose yield of 330–530 g/kg BW was obtained, and the sugars released were utilised for BDO production by E. ludwigii . The fed-batch cultivation using pure glucose and glucose rich hydrolysates from acid and enzymatic hydrolysis resulted in BDO titres of 144.5, 135.4, and 138.8 g/L, after 96 h, with yield of 0.47, 0.42 and 0.48 g/g yield, respectively. The innovation of the work is valorisation of BW to BDO with a circular biorefining approach and thus, reducing BW disposal and associated environmental burden.