ZmEHD1 Is Required for Kernel Development and Vegetative Growth through Regulating Auxin Homeostasis. Issue 3 (19th December 2019)

• Record Type: Journal Article
• Authors: Wang, Yafei; Liu, Wenwen; Wang, Hongqiu; Du, Qingguo; Fu, Zhiyuan; Li, Wen-Xue; Tang, Jihua
• Is Part Of: Plant physiology. Volume 182:Issue 3(2020)
• Page Start: 1467
• Page End: 1480
• Publication Date: 2019-12-19
• Subjects: Plant physiology -- Periodicals; Botany -- Periodicals; Periodicals; Electronic journals; 571.2
• DOI: 10.1104/pp.19.01336
• Languages: English
• ISSNs: 0032-0889

Abstract

Abstract : ZmEHD1 regulates auxin homeostasis by mediating clathrin-mediated endocytosis and is crucial for maize normal development. Abstract: The roles of C-terminal Eps15 homology domain (EHD) proteins in clathrin-mediated endocytosis in plants are poorly understood. Here, we isolated a maize ( Zea mays ) mutant, designated ehd1, which showed defects in kernel development and vegetative growth. Positional cloning and transgenic analysis revealed that ehd1 encodes an EHD protein. Internalization of the endocytic tracer FM4-64 was substantially reduced in the ehd1 mutant and ZmEHD1 knockout mutants. We further demonstrated that ZmEHD1 and the ZmAP2 σ subunit physically interact at the plasma membrane. Auxin distribution and ZmPIN1a-YFP localization were altered in the ehd1 mutant. Kernel indole-3-acetic acid levels were substantially lower in the ehd1 mutant than in wild-type maize. Exogenous application of 1-naphthaleneacetic acid, but not GA3 or 2-naphthaleneacetic acid, rescued the seed germination and seedling emergency phenotypic defects of ehd1 mutants. Taken together, these results indicate that ZmEHD1 regulates auxin homeostasis by mediating clathrin-mediated endocytosis through its interaction with the ZmAP2 σ subunit, which is crucial for kernel development and vegetative growth of maize.