MiR-122–5p regulates the pathogenesis of childhood obesity by targeting CPEB1. Issue 3 (May 2022)

• Record Type: Journal Article
• Authors: Li, Dawei; Chen, Jinni; Yun, Chuan; Li, Xiaoyan; Huang, Zhenzhen
• Is Part Of: Obesity research & clinical practice. Volume 16:Issue 3(2022)
• Page Start: 206
• Page End: 213
• Publication Date: 2022-05
• Subjects: Childhood obesity -- Inflammation -- MiR-122–5p -- CPEB1; Obesity -- Research -- Periodicals; Obesity -- Treatment -- Periodicals; Obesity -- Periodicals; Obésité -- Recherche -- Périodiques; Obésité -- Traitement -- Périodiques; Obesity -- Research; Obesity -- Treatment; Electronic journals; Periodicals; 616.398
• DOI: 10.1016/j.orcp.2022.05.006
• Languages: English
• ISSNs: 1871-403X

Abstract

Abstract: Background: Childhood obesity is strongly associated with inflammation which contributes to the development of several obesity-related disorders. Accumulating evidence has suggested that microRNAs (miRNAs) are involved in the pathogenesis of multiple human diseases, including childhood obesity. MiR-122–5p was reported to be related to obesity in childhood, however, the detailed function and mechanism of miR-122–5p are still obscure. Methods: Simpson-Golabi-Behmel syndrome (SGBS) adipocytes were cocultured with macrophage cell line THP-1 or macrophage-conditioned medium (MacCM) to promote cytokine expression. Oil Red O staining was used to detect the accumulation of lipid droplets in SGBS cells. The expression of interleukin 6 (IL-6), IL-8, and monocyte chemoattractant protein 1 (MCP-1) at mRNA and protein levels was assessed by RT-qPCR and ELISA, respectively. Western blotting was used for measuring protein levels of target genes of miR-122–5p. The luciferase reporter assay was applied for detecting the binding relation between miR-122–5p and cytoplasmic polyadenylation element binding protein 1 (CPEB1). Results: Coculture of SGBS adipocytes and THP-1 macrophages/MacCM promoted IL-6, IL-8, and MCP-1 expression at mRNA and protein levels. Overexpression of miR-122–5p inhibited IL-6, IL-8, and MCP-1 expression in SGBS adipocytes, and this inhibitory effect was rescued by CPEB1 upregulation. CPEB1 3′-untranslated region was directly targeted by miR-122–5p. Conclusion: MiR-122–5p suppresses cytokine expression in SGBS adipocytes by targeting CPEB1.