Metagenomics analysis unraveled the influence of sulfate radical-mediated compost nitrogen transformation process. (1st September 2022)

• Record Type: Journal Article
• Authors: Wang, Susu; Niu, Qiuqi; Zhu, Pengfei; Huang, Yite; Li, Kecheng; Li, Qunliang
• Is Part Of: Journal of environmental management. Volume 317(2022)
• Publication Date: 2022-09-01
• Subjects: Composting -- Nitrogen transformation -- Nitrification -- Denitrification -- SO− 4⋅; Environmental policy -- Periodicals; Environmental management -- Periodicals; Environment -- Periodicals; Ecology -- Periodicals; 363.705
• DOI: 10.1016/j.jenvman.2022.115436
• Languages: English
• ISSNs: 0301-4797

Abstract

Abstract: The mechanism of nitrogen transformation of sulfate radical (SO− 4⋅) in the process of composting is unclear. The objectives of this study were to investigate the influence of SO− 4⋅ on nitrogen biotransformation during composting and to compare the differences in physicochemical parameters and metagenomics analysis between CK (fresh dairy manure and bagasse pith) and PS (the composting raw materials added with potassium persulfate). The results indicated that SO − 4 ⋅ guides electron transfer in the conversion of NH + 4 -N to NO− 3-N and breaches the extracellular polysaccharide (EPS) structure to promote nitrogen removal. Aminomonooxygenase (AMO) and nitrate reductase (NR) levels displayed an interactive relationship between microorganisms and substrates. Metagenomics analysis revealed distinct microbial community compositions and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways between nitrification and denitrification. Correlation analysis indicated that Methanobrevibacter, Bacillus and Pseudomonas were closely related to these processes. This work demonstrates the effect of SO− 4⋅ on nitrogen cycling and retention, and possible mechanisms of nitrification and denitrification during composting. Graphical abstract: Image 1 Highlights: Effect of sulfate radical anion on nitrogen metabolism in composting was assessed. Microbial functions based on function gene database were predicted. Phyla were highly correlated with environmental factors during nitrogen conversion. Methanobrevibacter, Bacillus and Pseudomonas were vital to nitrogen transformation.