Chitosan-alginate nanoparticles as effective oral carriers to improve the stability, bioavailability, and cytotoxicity of curcumin diethyl disuccinate. (15th March 2021)

• Record Type: Journal Article
• Authors: Sorasitthiyanukarn, Feuangthit Niyamissara; Muangnoi, Chawanphat; Rojsitthisak, Pornchai; Rojsitthisak, Pranee
• Is Part Of: Carbohydrate polymers. Volume 256(2021)
• Publication Date: 2021-03-15
• Subjects: Curcumin diethyl disuccinate -- Chitosan-alginate nanoparticles -- Bioaccessibility -- Bioavailability; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.117426
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: Encapsulation of CDD in CANPs enhanced its stability during simulated digestion. CDD-CANPs exhibit a higher chemical stability during UV-light exposure. Encapsulation of CDD in CANPs increased its bioaccessibility during simulated digestion. Encapsulation of CDD in CANPs increased its bioavailability five-fold. CDD-CANPs had a higher cellular uptake and anticancer activity in HepG2 cells than free CDD. Abstract: Curcumin diethyl disuccinate (CDD) is an ester prodrug of curcumin that has better chemical stability in phosphate buffer (pH 7.4) and anticancer activities against MDA-MB-231 human breast cancer cells and Caco-2 cells than curcumin. However, a major drawback of CDD is its poor water solubility and low bioavailability in the gastrointestinal tract. To overcome these problems, a nanoformulation was developed using chitosan/alginate nanoparticles (CANPs) under the optimal condition as previously derived by statistical optimization. The CDD-loaded CANPs (CDD-CANPs) were found to exhibit good stability after exposure to simulated digestive fluids and ultraviolet light, and a sustained-release profile of CDD in the simulated digestive and body fluids. The in vitro release pattern fitted well to the Peppas-Sahlin model, indicating that the release of CDD was mainly governed by diffusion. Compared to free CDD, the CDD-CANPs showed better stability, bioaccessibility, bioavailability, cellular uptake, and cytotoxicity against HepG2 cells.