OFF bipolar cell density varies by subtype, eccentricity, and along the dorsal ventral axis in the mouse retina. Issue 8 (9th November 2020)

• Record Type: Journal Article
• Authors: Camerino, Michael J.; Engerbretson, Ian J.; Fife, Parker A.; Reynolds, Nathan B.; Berria, Mikel H.; Doyle, Jamie R.; Clemons, Mellisa R.; Gencarella, Michael D.; Borghuis, Bart G.; Fuerst, Peter G.
• Is Part Of: Journal of comparative neurology. Volume 529:Issue 8(2021)
• Page Start: 1911
• Page End: 1925
• Publication Date: 2020-11-09
• Subjects: axon -- dendrite -- development -- genetics -- neural -- neuron -- vision; Comparative neurobiology -- Periodicals; Neurology -- Periodicals; 616
• DOI: 10.1002/cne.25064
• Languages: English
• ISSNs: 0021-9967

Abstract

Abstract: The neural retina is organized along central‐peripheral, dorsal‐ventral, and laminar planes. Cellular density and distributions vary along the central‐peripheral and dorsal‐ventral axis in species including primates, mice, fish, and birds. Differential distribution of cell types within the retina is associated with sensitivity to different types of damage that underpin major retinal diseases, including macular degeneration and glaucoma. Normal variation in retinal distribution remains unreported for multiple cell types in widely used research models, including mouse. Here we map the distribution of all known OFF bipolar cell (BC) populations and horizontal cells. We report significant variation in the distribution of OFF BC populations and horizontal cells along the dorsal‐ventral and central‐peripheral axes of the retina. Distribution patterns are much more pronounced for some populations of OFF BC cells than others and may correspond to the cell type's specialized functions. Abstract : Density of OFF bipolar cells vary by subtype, eccentricity and along the dorsal ventral axis. (a) Retinas were disected into four quadrants labeled with short‐wave opsin to determine orientation. (b) Cells were marked and saved as series of individual coordinates which underwent Voronoi tessellation to analyze spatial heterogeneity. (c) Volumetric datasets were counted do determine cell count. (d) OFF‐bipolar cell density contour maps by subtype 1a, 1b, 2, 3a, 3b, 4.