Anti-breast cancer potential of MnO2 nanoparticles using Terminalia chebula fruit extract against MCF-7 cell line through in vitro cell cycle and apoptotic studies. (2022)

• Record Type: Journal Article
• Authors: Reddy, Pruthvish; Pradeep, Sushma; S M, Gopinath; Ramu, Ramith; Prasad Kollur, Shiva; Shivamallu, Chandan
• Is Part Of: Materials today. Volume 62:Part 8(2022)
• Page Start: 5526
• Page End: 5532
• Publication Date: 2022
• Subjects: Apoptogenic -- MCF7 -- Adenocarcinoma MnO2, -- Terminalia chebula; MnO2 NPs Manganese oxide nanoparticles -- MCF-7 Michigan Cancer Foundation-7 -- TC Terminalia chebula -- SEM scanning electron microscopy -- TEM transmission electron microscopy -- HR TEM - high-resolution transmission electron microscopy -- ABTS 2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid -- PBS Phosphate buffer saline -- ELISA Enzyme-linked immunoassay -- DMSO Dimethyl Sulfoxide -- nm nanometer -- MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide -- DMEM Dulbecco's Modified Eagle Medium -- rpm Revolutions per minute; Materials science -- Congresses -- Periodicals; 620.1
• DOI: 10.1016/j.matpr.2022.04.330
• Languages: English
• ISSNs: 2214-7853

Abstract

Abstract: Since decades, cancer has become reason of major concern as it is the leading cause of mortality worldwide. Many therapeutic strategies have come up in the scientific world, but its pitiful to know that synthetic chemotherapeutic agents either cause adverse effects or cancer cells develop resistance to these agents. Plant derived chemotherapeutic agent present wide range of therapeutics, and most are yet to be discovered. In the present study, we have analysed the anti-proliferative, and apoptogenic properties of manganese oxide nanoparticles MnO2 NPs derived from Terminalia chebula (TC) fruit on breast adenocarcinoma, MCF7 cell lines. The formation of MnO2 NPs was confirmed through scanning electron microscopy (SEM), transmission electron microscopy (TEM) and high-resolution transmission electron microscopy (HR-TEM). The morphology of the material was studied using SEM analysis, which suggested a agglomeration having spherical shape with an average diameter of 47.6 nm. Further, the TEM and HR-TEM images confirmed the rod shape of the as-prepared MnO2 NPs with an interplanar distance of 0.271 nm. The anticancer efficacy of MnO2 NPs was evaluated against MCF-7 breast cancer cell line, which showed up to 86% inhibition of the cells at 320 μg/mL concentration. The MnO2 NPs was found to be cytotoxic, and was able to induce apoptosis in MCF7 cell lines.