Late-day measurement of excised branches results in uncertainty in the estimation of two stomatal parameters derived from response curves in Populus deltoides Bartr. × Populus nigra L. (3rd February 2022)

• Record Type: Journal Article
• Authors: Davidson, Kenneth J; Lamour, Julien; Rogers, Alistair; Serbin, Shawn P
• Editors: Holtta, Teemu
• Is Part Of: Tree physiology. Volume 42:Number 7(2022)
• Page Start: 1377
• Page End: 1395
• Publication Date: 2022-02-03
• Subjects: climate model -- photosynthesis -- stomata -- stomatal conductance -- stomatal optimality -- stomatal response; Trees -- Physiology -- Periodicals; 582.16
• DOI: 10.1093/treephys/tpac006
• Languages: English
• ISSNs: 0829-318X

Abstract

Abstract: Many terrestrial biosphere models depend on an understanding of the relationship between stomatal conductance and photosynthesis. However, unlike the measurement of photosynthetic parameters, such as the maximum carboxylation capacity, where standard methods (e.g., CO2 response or AC i curves) are widely accepted, a consensus method for empirically measuring parameters representing stomatal response has not yet emerged. Most models of stomatal response to environment represent stomatal conductance as being bounded by a lower intercept parameter ( g 0 ), and linearly scaled based on a multivariate term described by the stomatal slope parameter ( g 1 ). Here we employ the widely used Unified Stomatal Optimization model, to test whether g 1 and g 0 parameters are impacted by the choice of measurement method, either on an intact branch or a cut branch segment stored in water. We measured paired stomatal response curves on intact and excised branches of a hybrid poplar clone ( Populus deltoides Bartr. × Populus nigra L. OP367), measured twice over a diurnal period. We found that predawn branch excision did not significantly affect measured g 0 and g 1 when measured within 4 h of excision. Measurement in the afternoon resulted in significantly higher values of g 1 and lower values of g 0, with values changing by 55% and 56%, respectively. Excision combined with afternoon measurement resulted in a marked effect on parameter estimates, with g 1 increasing 89% from morning to afternoon and a 25% lower g 1 for cut branches than those measured in situ. We also show that in hybrid poplar the differences in parameter estimates obtained from plants measured under different conditions can directly impact models of canopy function, reducing modeled transpiration by 18% over a simulated 12.5-h period. Although these results are only for a single isohydric woody species, our findings suggest that stomatal optimality parameters may not remain constant throughout the day.