Controllable assembly of synthetic constructs with programmable ternary DNA interaction. Issue 12 (17th June 2022)

• Record Type: Journal Article
• Authors: Cui, Huangchen; Zhang, Tianqing; Kong, Yuhan; Xing, Hang; Wei, Bryan
• Is Part Of: Nucleic acids research. Volume 50:Issue 12(2022)
• Page Start: 7188
• Page End: 7196
• Publication Date: 2022-06-17
• Subjects: Nucleic acids -- Periodicals; Molecular biology -- Periodicals; 572.805
• DOI: 10.1093/nar/gkac478
• Languages: English
• ISSNs: 0305-1048

Abstract

Abstract: Compared with the dual binding components in a binary interaction, the third component of a ternary interaction often serves as modulator or regulator in biochemical processes. Here, we presented a programmable ternary interaction strategy based on the natural DNA triplex structure. With the DNA triplex-based ternary interaction, we have successfully demonstrated controllable hierarchical assemblies from nanometer scale synthetic DNA nanostructure units to micrometer scale live bacteria. A selective signaling system responsive to orthogonal nucleic acid signals via ternary interaction was also demonstrated. This assembly method could further enrich the diversified design schemes of DNA nanotechnology.