Processing tomato chlorophyll a/b-binding protein 1C interacts with CMV 2b protein. (July 2022)

• Record Type: Journal Article
• Authors: Liu, Zhen; Yu, Chengmin; Xiang, Benchun; Niu, Jianxin; Zheng, Yinying
• Is Part Of: Physiological and molecular plant pathology. Volume 120(2022)
• Publication Date: 2022-07
• Subjects: Processing tomato -- Cucumber mosaic virus -- Protein interactions -- Silencing suppressor -- Viral infection; Cucumber mosaic virus CMV -- Chlorophyll a/b-binding protein Cab -- Light-harvesting chlorophyll a/b-binding proteins LHC; Plant diseases -- Periodicals; Diseased plants -- Physiology -- Periodicals; Phytopathogenic microorganisms -- Host plants -- Periodicals; 632
• DOI: 10.1016/j.pmpp.2022.101857
• Languages: English
• ISSNs: 0885-5765

Abstract

Abstract: Light-harvesting chlorophyll a /b-binding proteins (LHCs) are nuclear-encoded thylakoid membrane proteins. In tomatoes, the Cab-1C gene encodes the type I Lhcb1 protein in LHCII. The GAL4 yeast two-hybrid system and BiFC experiments showed an interaction between SlCab-1C of processing tomato and the CMV 2b protein. At 56 dpi, the accumulation of CMV CP in the apical leaves of transgenic tobacco overexpressing the processing tomato Cab-1C gene was significantly reduced compared to wild-type tobacco. In addition, the interaction region between CMV 2b and SlCab-1C was located at amino acids 43–184 of the SlCab-1C protein. Highlights: SlCab-1C and CMV 2b interact in plants. The overexpression of SlCab-1C affects CMV CP accumulation in tobacco leaves. The region where SlCab-1C interacts with CMV 2b is located at amino acids 43–184 of the SlCab-1C protein.