On the role of skeletal muscle acidosis and inorganic phosphates as determinants of central and peripheral fatigue: A 31P‐MRS study. (2nd June 2022)

• Record Type: Journal Article
• Authors: Hureau, Thomas J.; Broxterman, Ryan M.; Weavil, Joshua C.; Lewis, Matthew T.; Layec, Gwenael; Amann, Markus
• Is Part Of: Journal of physiology. Volume 600:Number 13(2022)
• Page Start: 3069
• Page End: 3081
• Publication Date: 2022-06-02
• Subjects: exercise -- intramuscular metabolic by‐product -- magnetic resonance spectroscopy -- neuromuscular fatigue; Physiology -- Periodicals; 612.005
• DOI: 10.1113/JP283036
• Languages: English
• ISSNs: 0022-3751

Abstract

Abstract : Abstract: Intramuscular hydrogen ion (H + ) and inorganic phosphate (Pi) concentrations were dissociated during exercise to challenge their relationships with peripheral and central fatigue in vivo . Ten recreationally active, healthy men (27 ± 5 years; 180 ± 4 cm; 76 ± 10 kg) performed two consecutive intermittent isometric single‐leg knee‐extensor trials (60 maximal voluntary contractions; 3 s contraction, 2 s relaxation) interspersed with 5 min of rest. Phosphorus magnetic resonance spectroscopy ( 31 P‐MRS) was used to continuously quantify intramuscular [H + ] and [Pi] during both trials. Using electrical femoral nerve stimulation, quadriceps twitch force ( Q tw ) and voluntary activation (VA) were quantified at rest and throughout both trials. Decreases in Q tw and VA from baseline were used to determine peripheral and central fatigue, respectively. Q tw was strongly related to both [H + ] (β coefficient: −0.9, P < 0.0001) and [Pi] (−1.1, P < 0.0001) across trials. There was an effect of trial on the relationship between Q tw and [H + ] (−0.5, P < 0.0001), but not Q tw and [Pi] (0.0, P = 0.976). This suggests that, unlike the unaltered association with [Pi], a given level of peripheral fatigue was associated with a different [H + ] in Trial 1 vs . Trial 2. VA was related to [H + ] (−0.3, P < 0.0001), but not [Pi] (−0.2, P = 0.243), across trials and there was no effect of trial (−0.1, P = 0.483). Taken together, these results support intramuscular Pi as a primary cause of peripheral fatigue, and muscle acidosis, probably acting on group III/IV muscle afferents in the interstitial space, as a contributor to central fatigue during exercise. Key points: We investigated the relationship between intramuscular metabolites and neuromuscular function in humans performing two maximal, intermittent, knee‐extension trials interspersed with 5 min of rest. Concomitant measurements of intramuscular hydrogen (H + ) and inorganic phosphate (Pi) concentrations, as well as quadriceps twitch‐force ( Q tw ) and voluntary activation (VA), were made throughout each trial using phosphorus magnetic resonance spectroscopy ( 31 P‐MRS) and electrical femoral nerve stimulations. Although [Pi] fully recovered prior to the onset of the second trial, [H + ] did not. Q tw was strongly related to both [H + ] and [Pi] across both trials. However, the relationship between Q tw and [H + ] shifted leftward from the first to the second trial, whereas the relationship between Q tw and [Pi] remained unaltered. VA was related to [H + ], but not [Pi], across both trials. These in vivo findings support the hypotheses of intramuscular Pi as a primary cause of peripheral fatigue, and muscle acidosis, probably acting on group III/IV muscle afferents, as a contributor to central fatigue. Abstract : Abstract figure legend Schematic diagram illustrating the experimental design and major findings.