Effects of aging and inflammation on catalase activity in human dental pulp. (September 2022)

• Record Type: Journal Article
• Authors: Aslantas, Eda Ezgi; Buzoglu, Hatice Dogan; Muftuoglu, Sevda Fatma; Atilla, Pergin; Karapinar, Senem Pinar; Aksoy, Yasemin
• Is Part Of: Archives of oral biology. Volume 141(2022)
• Publication Date: 2022-09
• Subjects: Aging -- Catalase -- Dental pulp -- Inflammation -- Pulpitis; Mouth -- Periodicals; Mouth -- Diseases -- Periodicals; Dentistry -- Periodicals; Electronic journals; 617.6005
• DOI: 10.1016/j.archoralbio.2022.105482
• Languages: English
• ISSNs: 0003-9969

Abstract

Abstract: Objectives: In this study, it was aimed to examine the catalase activity in clinically healthy and caries-related inflamed symptomatic human dental pulps of young and old individuals. Design: Sixty pulp samples from young and old healthy donors, were collected depending on pulpal status. 48 samples were used for spectrophotometric analysis and 12 samples for immunohistochemistry. Healthy pulps were maintained from non-carries, extracted third molars. Reversible and irreversible pulpitis samples were obtained by pulp extirpation during endodontic treatment. Following homogenization catalase enzyme activity was determined by spectrophotometry. Additionally, two pulp tissue samples from each group were fixed and evaluated for catalase immunoreactivity. Results: Catalase enzyme activity in old healthy pulp samples were significantly higher than healthy young samples (p ≤ 0.05). Reversible and irreversible pulpitis samples indicated significantly decreased activity compared to healthy samples in elderly group (p ≤ 0.05). Young reversible pulpitis samples showed significantly increased catalase activity when compared to irreversible pulpitis and the reversible pulpitis samples in elderly group (p ≤ 0.05). Immunohistochemical evaluation indicated that there was intense catalase immunoreactivity in young patients with reversible pulpitis compared with reversible pulpitis in elderly group. However, weak immunoreactivity was observed in young irreversible pulpitis and elderly reversible pulpitis samples. Conclusions: The pulp tissues presented different levels of catalase activities against pulpitis and aging. Highlights: Pulpal status and aging can increase free radical formation of dental pulp. Catalase is one of the antioxidant defense system suppresses free radical formation. Aging may affect catalase enzyme activity. Dental pulp creates different levels of catalase activities against inflammation.