Giardia lamblia regulates the production of proinflammatory cytokines through activating the NOD2–Rip2–ROS signaling pathway in mouse macrophages. Issue 6 (16th May 2022)

• Record Type: Journal Article
• Authors: Zhang, Xu; Li, Xin; Yu, Yanhui; Zhang, Xichen; Wang, Xiaocen; Zhang, Nan; Chen, Mengge; Gong, Pengtao; Li, Jianhua
• Is Part Of: Immunology and cell biology. Volume 100:Issue 6(2022)
• Page Start: 440
• Page End: 452
• Publication Date: 2022-05-16
• Subjects: G. lamblia -- NOD2–Rip2 -- proinflammatory cytokines -- ROS; Immunology -- Periodicals; Cytology -- Periodicals; 616.079
• DOI: 10.1111/imcb.12550
• Languages: English
• ISSNs: 0818-9641

Abstract

Abstract: Giardia lamblia is a zoonotic protozoan that causes the diarrheal illness giardiasis, with the highest prevalence reported in the tropics and subtropics. Giardia is currently the most frequently identified pathogen in waterborne outbreaks in the United States. Nucleotide oligomerization domain (NOD) 1 and NOD2, intracellular NOD‐like receptors, recognize pathogens to induce proinflammatory and antimicrobial responses. However, the roles of NOD1 and NOD2 signaling in Giardia infection have not yet been investigated. In the present study, the activation of NOD1 and NOD2 signaling pathways and the production of proinflammatory cytokines, reactive oxygen species (ROS) and nitric oxide in mouse macrophages stimulated with G. lamblia or parasite excretory–secretory products (ESPs) were examined. The results showed that G. lamblia and ESPs activated NOD2 and its downstream adaptor protein kinase, Receptor‐interacting protein 2 (Rip2), in mouse macrophages. Blocking NOD2–Rip2 signaling significantly reduced the production of ROS and subsequently decreased the phosphorylation of nuclear factor‐κB p65 and extracellular signal‐regulated kinase, which in turn inhibited the production of four proinflammatory cytokines, namely, interleukin (IL)‐1β, IL‐6, IL‐12p40 and tumor necrosis factor‐α. In summary, our results indicate that the NOD2–Rip2 signal, which is activated by G. lamblia, contributes to the production of proinflammatory cytokines and ROS in mouse macrophages. Abstract : Giardia lamblia could produce excretory–secretory products to activate the Nucleotide oligomerization domain 2 (NOD2)–receptor‐interacting protein 2 (Rip2) signal, promoting reactive oxygen species (ROS) production and leading to the phosphorylation of nuclear factor‐kappa B (NF‐κB) p65 and extracellular signal‐regulated kinase (ERK), which in turn regulated the production of proinflammatory cytokines in mouse macrophages. In addition, G. lamblia ‐induced ROS production was involved in ERK and NF‐κB pathway activation in mouse macrophages.