A Fluorescent Cage for Supramolecular Sensing of 3‐Nitrotyrosine in Human Blood Serum. Issue 28 (23rd May 2022)

• Record Type: Journal Article
• Authors: Pérez‐Márquez, Lidia A.; Perretti, Marcelle D.; García‐Rodríguez, Raúl; Lahoz, Fernando; Carrillo, Romen
• Is Part Of: Angewandte Chemie international edition. Volume 61:Issue 28(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-05-23
• Subjects: Molecular Cages -- Molecular Recognition -- Nitrotyrosine -- Sensors -- Tetraphenylethene; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.202205403
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: 3‐Nitrotyrosine (NT) is generated by the action of peroxynitrite and other reactive nitrogen species (RNS), and as a consequence it is accumulated in inflammation‐associated conditions. This is particularly relevant in kidney disease, where NT concentration in blood is considerably high. Therefore, NT is a crucial biomarker of renal damage, although it has been underestimated in clinical diagnosis due to the lack of an appropriate sensing method. Herein we report the first fluorescent supramolecular sensor for such a relevant compound: Fluorescence by rotational restriction of tetraphenylethenes (TPE) in a covalent cage is selectively quenched in human blood serum by 3‐nitrotyrosine (NT) that binds to the cage with high affinity, allowing a limit of detection within the reported physiological concentrations of NT in chronic kidney disease. Abstract : Fluorescence by rotational restriction of two tetraphenylethenes (TPE) in a covalent cage is selectively quenched in human blood serum by 3‐nitrotyrosine, a key biomarker of kidney injury. This cage, with a limit of detection under physiologically relevant concentrations, is the first reported fluorescent supramolecular sensor for such a relevant compound.