Expression of anti-NbHK single-chain antibody in fusion with NSlmb enhances the resistance to Nosema bombycis in Sf9-III cells. Issue 4 (6th August 2022)

• Record Type: Journal Article
• Authors: Zhang, Renze; Zheng, Shiyi; Huang, Hongyun; Sun, Xi; Huang, Yukang; Wei, Junhong; Pan, Guoqing; Li, Chunfeng; Zhou, Zeyang
• Is Part Of: Bulletin of entomological research. Volume 112:Issue 4(2022)
• Page Start: 502
• Page End: 508
• Publication Date: 2022-08-06
• Subjects: Hexokinase -- Nosema bombycis -- resistant -- single-chain antibody -- ubiquitination system; Entomology -- Periodicals; 595.7
• DOI: 10.1017/S0007485321001036
• Languages: English
• ISSNs: 0007-4853

Abstract

Abstract: Nosema bombycis is a destructive and specific intracellular parasite of silkworm, which is extremely harmful to the silkworm industry. N. bombycis is considered as a quarantine pathogen of sericulture because of its long incubation period and horizontal and vertical transmission. Herein, two single-chain antibodies targeting N. bombycis hexokinase (NbHK) were cloned and expressed in fusion with the N-terminal of Slmb (a Drosophila melanogaster FBP), which contains the F-box domain. Western blotting demonstrated that Sf9-III cells expressed NSlmb–scFv-7A and NSlmb–scFv-6H, which recognized native NbHK. Subsequently, the NbHK was degraded by host ubiquitination system. When challenged with N. bombycis, the transfected Sf9-III cells exhibited better resistance relative to the controls, demonstrating that NbHK is a prospective target for parasite controls and this approach represents a potential solution for constructing N. bombycis -resistant Bombyx mori .