Design and Synthesis of Glycosylated Cholera Toxin B Subunit as a Tracer of Glycoprotein Trafficking in Organelles of Living Cells. Issue 37 (23rd May 2022)

• Record Type: Journal Article
• Authors: Maki, Yuta; Kawata, Kazuki; Liu, Yanbo; Goo, Kang‐Ying; Okamoto, Ryo; Kajihara, Yasuhiro; Satoh, Ayano
• Is Part Of: Chemistry. Volume 28:Issue 37(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-05-23
• Subjects: glycoprotein -- N-glycan -- cholera toxin -- native chemical ligation -- live imaging; Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.202201253
• Languages: English
• ISSNs: 0947-6539

Abstract

Abstract: Glycosylation of proteins is known to be essential for changing biological activity and stability of glycoproteins on the cell surfaces and in body fluids. Delivering of homogeneous glycoproteins into the endoplasmic reticulum (ER) and the Golgi apparatus would enable us to investigate the function of asparagine‐linked ( N ‐) glycans in the organelles. In this work, we designed and synthesized an intentionally glycosylated cholera toxin B‐subunit (CTB) to be transported to the organelles of mammalian cells. The heptasaccharide, the intermediate structure of various complex‐type N ‐glycans, was introduced to the CTB. The synthesized monomeric glycosyl‐CTB successfully entered mammalian cells and was transported to the Golgi and the ER, suggesting the potential use of synthetic CTB to deliver and investigate the functions of homogeneous N ‐glycans in specific organelles of living cells. Abstract : We designed and chemically synthesized intentionally glycosylated cholera toxin B‐subunit (glycosyl‐CTB) as a tracer to investigate maturation and functions of N ‐glycans on the biosynthesis of glycoproteins. The obtained glycoprotein was successfully transported to the endoplasmic reticulum (ER) and the Golgi apparatus of living cells.