The structural effects of Vitamin A deficiency on biological macromolecules due to ethanol consumption and withdrawal: An FTIR study with chemometrics. Issue 7 (11th April 2022)

• Record Type: Journal Article
• Authors: Elibol, Birsen; Severcan, Mete; Jakubowska‐Dogru, Ewa; Dursun, Ilknur; Severcan, Feride
• Is Part Of: Journal of biophotonics. Volume 15:Issue 7(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-04-11
• Subjects: ethanol -- FT‐IR spectroscopy -- male rat hippocampus -- vitamin a deficiency; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.202100377
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract: The structural effects of vitamin A‐deficiency were examined on the molecular profiles of biomolecules of male rat hippocampus during prolonged ethanol intake/withdrawal using FT‐IR spectroscopy coupled with chemometrics. Liquid ethanol diet with/without vitamin A was maintained to adult rats for 3‐months. The rats were decapitated at different ethanol withdrawal times and FT‐IR spectra were obtained. Ethanol consumption/withdrawal produced significant changes in proteins' conformations, while having insignificant structural effects on lipids. In vitamin A deficiency, ethanol produced structural changes in lipids by lipid ordering especially in the early‐ethanol withdrawal. Furthermore, an increase in lipid and protein content, saturated/unsaturated lipid ratio, a decrease in nucleic acids content and decrease in membrane fluidity were observed. These changes were less severe in the presence of Vitamin A. This study is clinically important for individuals with vitamin A deficiency because they have to be more cautious when consuming alcohol to protect themselves from cognitive dysfunctions. Abstract : The deteriorating effects of vitamin A deficiency on the hippocampal molecular profile of chronic alcoholic individuals were demonstrated using FTIR spectroscopy with chemometric methods such as PCA and SIMCA. Significant changes in the structure and amount of lipids, proteins and nucleic acids were observed in ethanol consumption and withdrawal accompanied with vitamin A deficient diet. The beneficial effects of vitamin A in the diet were also shown by the corrective capacity of vitamin A for ethanol‐related hippocampal damage.