Β‐Glucosidase produced by Moniliophthora perniciosa: Characterization and application in the hydrolysis of sugarcane bagasse. (2nd May 2021)

• Record Type: Journal Article
• Authors: Almeida, Larissa Emanuelle da Silva; Ribeiro, Geise Camila Araújo; Aparecida de Assis, Sandra
• Is Part Of: Biotechnology and applied biochemistry. Volume 69:Number 3(2022)
• Page Start: 963
• Page End: 973
• Publication Date: 2021-05-02
• Subjects: biomass pretreatment -- enzymatic reactions -- fermentation -- fungal -- lignocellulose -- Moniliophthora perniciosa; Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.2167
• Languages: English
• ISSNs: 0885-4513

Abstract

Abstract: β‐Glucosidases (BGLs) belong to the group of enzymes of cellulases and act in the last stage of cellulose degradation, releasing glucose molecules, eliminating the inhibitory effect of cellobiose. This study focused on the production, characterization, and application of BGL from Moniliophthora perniciosa in the hydrolysis of pretreated sugarcane bagasse (3% NaOH + 6% Na2 SO3 ), with varying enzymatic loads and reaction times. The enzyme showed an optimum pH of 4.5 and 60°C. It was stable at all temperatures analyzed (50–90°C) and retained about 100% of its activity at 50°C after 60 min of incubation. Among the ions analyzed, BaCl2 increased BGL activity 9.04 ± 1.41 times. The maximum production of reducing sugars (89.15%) was achieved after 48 h with 10 mg of protein. Abstract : β‐glucosidase was produced by submerged fermentation of Moniliophthora perniciosa and applied in the hydrolysis sugarcane bagasse