A novel streptonigrin type alkaloid from the Streptomyces flocculus CGMCC 4.1223 mutant ΔstnA/Q2. Issue 13 (1st July 2022)

• Record Type: Journal Article
• Authors: Wang, Xiaozheng; Xu, Fei; Huang, Tingting; Deng, Zixin; Lin, Shuangjun
• Is Part Of: Natural product research. Volume 36:Issue 13(2022)
• Page Start: 3337
• Page End: 3345
• Publication Date: 2022-07-01
• Subjects: Streptomyces flocculus -- streptonigrin -- streptonigramide biosynthesis; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2020.1856840
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: Streptonigrin (STN) is a highly functionalized aminoquinone alkaloid with broad and potent antitumor activities. Previously, the biosynthetic gene cluster of STN was identified in Streptomyces flocculus CGMCC 4.1223, revealing an α/β-hydrolase (StnA) and a methyltransferase (StnQ2). In this work, a double mutant Δ stnA/Q2 was constructed by genetic manipulation and produced a novel derivative of STN, named as streptonigramide. Structure of streptonigramide was established by spectroscopic analyses. Its biosynthetic pathway has been proposed as well. Graphical Abstract: UF0001