AMP-activated protein kinase mediates glycolysis in post-mortem breast muscle of broilers. Issue 1 (31st December 2022)

• Record Type: Journal Article
• Authors: Ruixia, Lan; Wei, Linlin; Wang, Yuchen; Wu, Fan
• Is Part Of: Italian journal of animal science. Volume 21:Issue 1(2022)
• Page Start: 1067
• Page End: 1073
• Publication Date: 2022-12-31
• Subjects: Chicken -- AMPK -- post-mortem muscle glycolysis -- Compound C -- AIRCR; Animal culture -- Periodicals; Livestock -- Italy -- Periodicals; Veterinary medicine -- Italy -- Periodicals; Animal culture; Livestock; Veterinary medicine; Italy; Periodicals; Periodicals; 636.005
• DOI: 10.1080/1828051X.2022.2093138
• Languages: English
• ISSNs: 1828-051X

Abstract

Abstract: The aim of this study was to confirm the role of AMP-activated protein kinase (AMPK) in the regulation of post-mortem breast muscle glycolysis in the broiler, we evaluated the effects of intraperitoneal injection of the specific AMPK activator (5-aminoimidazole-4-carboxamide ribonucleoside, AICAR) and inhibitor (6-[4-(2-piperidin-1-yl-ethoxy)-phenyl]-3-pyridin-4-yl-pyrazolo[1, 5-a] pyrimidine, Compound C) on AMPK phosphorylation (p-AMPK) level, meat quality, glycolytic metabolites and glycolytic enzymes activities in post-mortem breast muscle of broilers. Compared to the CON group, intraperitoneal injection of AIRCR increased p-AMPK level, glycolytic enzyme activities and lactate content in post-mortem breast muscle, increased lightness ( L* ) and drip loss, but decreased pH45min and pH24h . However, intraperitoneal injection of Compound C, decreased p-AMPK level, glycolytic enzyme activities, glucose-6-phosphate and lactate content in post-mortem breast muscle, and increased pH24h . Based on these results, we conclude that AMPK played a vital role in regulating post-mortem glycolysis in the breast muscle of broilers. HIGHLIGHTS: Injection of AIRCR promoting muscle glycolysis. Injection of Compound C alleviating muscle glycolysis. AMPK played a vital role in regulating post-mortem muscle glycolysis.