MiRNA-296-5p functions as a potential tumor suppressor in human osteosarcoma by targeting SND1. Issue 5 (5th March 2021)

• Record Type: Journal Article
• Authors: Huang, Ya-Zeng; Zhang, Jun; Shen, Jian-Jian; Zhao, Ting-Xiao; Xu, You-Jia
• Editors: Wang, Ning-Ning
• Is Part Of: Chinese medical journal. Volume 134:Issue 5(2021)
• Page Start: 564
• Page End: 572
• Publication Date: 2021-03-05
• Subjects: miR-296-5p -- Osteosarcoma -- SND1 -- Tumor suppressor -- miRNA; Medicine -- Periodicals; Medicine, Oriental -- Periodicals; Medicine; Medicine, Oriental; Medicine; Medicine, East Asian Traditional; Periodicals; Electronic journals; 610.5
• DOI: 10.1097/CM9.0000000000001400
• Languages: English
• ISSNs: 0366-6999

Abstract

Abstract: Background: The pathogenesis of osteosarcoma (OS) is still unclear, and it is still necessary to find new targets and drugs for anti-OS. This study aimed to investigate the role and mechanism of the anti-OS effects of miR-296-5p. Methods: We measured the expression of miR-296-5p in human OS cell lines and tissues. The effect of miR-296-5p and its target gene staphylococcal nuclease and tudor domain containing 1 on proliferation, migration, and invasion of human OS lines was examined. The Student's t test was used for statistical analysis. Results: We found that microRNA (miR)-296-5p was significantly downregulated in OS cell lines and tissues (control vs . OS, 1.802 ± 0.313 vs . 0.618 ± 0.235, t = 6.402, P < 0.01). Overexpression of miR-296-5p suppressed proliferation, migration, and invasion of OA cells. SND1 was identified as a target of miR-296-5p by bioinformatic analysis and dual-luciferase reporter assay. Overexpression of SND1 abrogated the effects induced by miR-296-5p upregulation (miRNA-296-5p vs . miRNA-296-5p + SND1, 0.294 ± 0.159 vs . 2.300 ± 0.277, t = 12.68, P = 0.003). Conclusion: Our study indicates that miR-296-5p may function as a tumor suppressor by targeting SND1 in OS.