Rapid authentication of stingless bees (Heterotrigona itama) honey by UV spectroscopy and hierarchical cluster analysis. Issue 1 (1st May 2022)

• Record Type: Journal Article
• Authors: Suhandy, D; Yulia, M; Kusumiyati,
• Is Part Of: IOP conference series. Volume 1024:Issue 1(2022)
• Publication Date: 2022-05-01
• Subjects: Earth sciences -- Periodicals; Environmental sciences -- Congresses; Environmental sciences -- Periodicals; 550.5
• DOI: 10.1088/1755-1315/1024/1/012064
• Languages: English
• ISSNs: 1755-1307

Abstract

Abstract: The price of stingless bee ( Heterotrigona itama ) honey is almost twice higher than the one produced by a non-stingless bee ( Apis mellifera ) due to its limited production and high content of polyphenol and flavonoids compounds. However, in the market, the authentication of stingless bees and non-stingless bees honey becomes difficult due to the similarity in the color of honey and its sugar content. In this research, we propose a simple analytical approach by combining ultraviolet (UV) spectroscopy and hierarchical cluster analysis (HCA) for discrimination between stingless and non-stingless bee honey. Fifty samples of monofloral stingless bee ( Heterotrigona itama ) honey and non-stingless bee ( Apis mellifera ) honey from Acacia mangium was used. The samples were diluted with a distilled water at a proportion of 1:30 (volume/volume). The spectral data were acquired in the range of 190-1100 nm using a benchtop UV-Vis spectrometer with fast scanning mode. The HCA was applied for selected preprocessed spectral data in the range of 230-400 nm. The result shows that HCA could be effective to discriminate between stingless and non-stingless bee honey. Shortly, it is expected to realize the simple and quick analytical method to authenticate stingless bee honey based on UV spectroscopy and the HCA method.