Pseudomonas sp. NGC7 as a microbial chassis for glucose-free muconate production from a variety of lignin-derived aromatics and its application to the production from sugar cane bagasse alkaline extract. (September 2022)

• Record Type: Journal Article
• Authors: Akutsu, Miho; Abe, Nanase; Sakamoto, Chiho; Kurimoto, Yuki; Sugita, Haruka; Tanaka, Makoto; Higuchi, Yudai; Sakamoto, Kimitoshi; Kamimura, Naofumi; Kurihara, Hiroyuki; Masai, Eiji; Sonoki, Tomonori
• Is Part Of: Bioresource technology. Volume 359(2022)
• Publication Date: 2022-09
• Subjects: cis, cis-Muconate -- Sugar cane bagasse -- Alkaline extracts -- Lignin -- Pseudomonas; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.127479
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Pseudomonas sp. NGC7 was used as a host cell to produce muconate. Muconate was produced from a variety of aromatics by tuning its metabolic flow. The engineered strains produced muconate without using carbon other than aromatics. Glucose-free muconate production from bagasse alkaline extract was demonstrated. Abstract: cis, cis -Muconate (ccMA) is a promising platform for use in synthesizing various polymers. A glucose-free ccMA production using Pseudomonas sp. NGC7 from hardwood lignin-derived aromatic compounds was previously reported. In that system, syringyl nucleus compounds were essential for growth. Here, it is shown that NGC7 is available for glucose-free ccMA production even from a mixture of lignin-derived aromatics that does not contain syringyl nucleus compounds. By introducing a gene set for the protocatechuate (PCA)-shunt consisting of PCA 3, 4-dioxygenase and PCA decarboxylase into an NGC7-derived strain deficient in PCA 3, 4-dioxygenase and ccMA cycloisomerase, it was succeeded in constructing a ccMA-producing strain that grows on a lignin-derived aromatics mixture containing no syringyl nucleus compounds. Finally, it is demonstrated that the engineered strain produced ccMA from sugar cane bagasse alkaline extract in 18.7 mol%. NGC7 is thus shown to be a promising microbial chassis for biochemicals production from lignin-derived aromatics.