CircNup188/miR-760–3p/Map3k8 axis regulates inflammation in cerebral ischemia. (August 2022)
- Record Type:
- Journal Article
- Title:
- CircNup188/miR-760–3p/Map3k8 axis regulates inflammation in cerebral ischemia. (August 2022)
- Main Title:
- CircNup188/miR-760–3p/Map3k8 axis regulates inflammation in cerebral ischemia
- Authors:
- Zhang, Hui
Deng, Jian
Huang, Kangling
He, Yibo
Cai, Zhili
He, Yitao - Abstract:
- Abstract: Introduction: Cerebral ischemia is a serious acute disease with high mortality and disability rates. circRNAs are associated with cerebral ischemia inflammation and can be used as therapeutic targets. We aimed to investigate circNup188/miR-760–3p/Map3k8 role in inflammation during cerebral ischemia. Methods: According to the sequencing results of previously published, signaling pathways and circRNAs related to inflammation were screened and looped for verification. In vitro OGD/R cell model was constructed to detect OGD/R effects on PC12 cell viability, apoptosis, inflammatory cytokines TNF-α, IL-1β and IL-6. qRT-PCR assessed circRNAs, circNup188 bound miRNAs, and four mRNAs associated with inflammation and brain diseases expressions. p65 and IkB-α and their phosphorylated proteins in NF-κB pathway and Map3k8 expressions were assessed by Western blot. Results: KEGG analysis revealed MAPK and NF-kappaB signaling pathways played a vital role in it, and were classic inflammatory pathways. circNup188, circU2, and circCnot2 were verified to be the loop structure. circNup188 might play an essential role in OGD/R cell model. Regulating circNup188 expression could affect OGD/R cells function. In addition, miR-760–3p might play a vital role in OGD/R cell model. Moreover, circNup188 regulated cerebral ischemia by sponging miR-760–3p. miR-760–3p might be involved in inflammation in OGD/R cell model by regulating Map3k8 to activate the NF-κB pathway. Conclusions: circNup188Abstract: Introduction: Cerebral ischemia is a serious acute disease with high mortality and disability rates. circRNAs are associated with cerebral ischemia inflammation and can be used as therapeutic targets. We aimed to investigate circNup188/miR-760–3p/Map3k8 role in inflammation during cerebral ischemia. Methods: According to the sequencing results of previously published, signaling pathways and circRNAs related to inflammation were screened and looped for verification. In vitro OGD/R cell model was constructed to detect OGD/R effects on PC12 cell viability, apoptosis, inflammatory cytokines TNF-α, IL-1β and IL-6. qRT-PCR assessed circRNAs, circNup188 bound miRNAs, and four mRNAs associated with inflammation and brain diseases expressions. p65 and IkB-α and their phosphorylated proteins in NF-κB pathway and Map3k8 expressions were assessed by Western blot. Results: KEGG analysis revealed MAPK and NF-kappaB signaling pathways played a vital role in it, and were classic inflammatory pathways. circNup188, circU2, and circCnot2 were verified to be the loop structure. circNup188 might play an essential role in OGD/R cell model. Regulating circNup188 expression could affect OGD/R cells function. In addition, miR-760–3p might play a vital role in OGD/R cell model. Moreover, circNup188 regulated cerebral ischemia by sponging miR-760–3p. miR-760–3p might be involved in inflammation in OGD/R cell model by regulating Map3k8 to activate the NF-κB pathway. Conclusions: circNup188 might up-regulate Map3k8 expression through sponging miR-760–3p and then activate NF-κB pathway, which was involved in cerebral ischemia development. This study provided a new target for cerebral ischemia treatment. Highlights: Regulating circNup188 expression could affect OGD/R cells function. CircNup188 regulated cerebral ischemia by sponging miR-760–3p. MiR-760–3p might be involved in inflammation in OGD/R cell model by regulating Map3k8 to activate the NF-κB pathway. … (more)
- Is Part Of:
- Molecular and cellular probes. Volume 64(2022)
- Journal:
- Molecular and cellular probes
- Issue:
- Volume 64(2022)
- Issue Display:
- Volume 64, Issue 2022 (2022)
- Year:
- 2022
- Volume:
- 64
- Issue:
- 2022
- Issue Sort Value:
- 2022-0064-2022-0000
- Page Start:
- Page End:
- Publication Date:
- 2022-08
- Subjects:
- circNup188 -- miR-760–3p -- Map3k8 -- Cerebral ischemia -- Inflammation
Molecular probes -- Diagnostic use -- Periodicals
Pathology, Cellular -- Technique -- Periodicals
Cell Biology -- Periodicals
Molecular Biology -- Periodicals
Sondes moléculaires -- Utilisation diagnostique -- Périodiques
Cytopathologie -- Technique -- Périodiques
572 - Journal URLs:
- http://www.sciencedirect.com/science/journal/08908508 ↗
http://firstsearch.oclc.org ↗
http://firstsearch.oclc.org/journal=0890-8508;screen=info;ECOIP ↗
http://www.elsevier.com/journals ↗ - DOI:
- 10.1016/j.mcp.2022.101830 ↗
- Languages:
- English
- ISSNs:
- 0890-8508
- Deposit Type:
- Legaldeposit
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- Available online (eLD content is only available in our Reading Rooms) ↗
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- British Library DSC - 5900.761000
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