Constitutive Expression of Arabidopsis SMALL AUXIN UP RNA19 (SAUR19) in Tomato Confers Auxin-Independent Hypocotyl Elongation. Issue 2 (20th December 2016)

• Record Type: Journal Article
• Authors: Spartz, Angela K.; Lor, Vai S.; Ren, Hong; Olszewski, Neil E.; Miller, Nathan D.; Wu, Guosheng; Spalding, Edgar P.; Gray, William M.
• Is Part Of: Plant physiology. Volume 173:Issue 2(2017)
• Page Start: 1453
• Page End: 1462
• Publication Date: 2016-12-20
• Subjects: Plant physiology -- Periodicals; Botany -- Periodicals; Periodicals; Electronic journals; 571.2
• DOI: 10.1104/pp.16.01514
• Languages: English
• ISSNs: 0032-0889

Abstract

Abstract : Constitutive expression of SAUR genes increases cell wall extensibility and promotes auxin-independent elongation growth in Arabidopsis and tomato. Abstract: The plant hormone indole-3-acetic acid (IAA or auxin) mediates the elongation growth of shoot tissues by promoting cell expansion. According to the acid growth theory proposed in the 1970s, auxin activates plasma membrane H + -ATPases (PM H + -ATPases) to facilitate cell expansion by both loosening the cell wall through acidification and promoting solute uptake. Mechanistically, however, this process is poorly understood. Recent findings in Arabidopsis ( Arabidopsis thaliana ) have demonstrated that auxin-induced SMALL AUXIN UP RNA ( SAUR ) genes promote elongation growth and play a key role in PM H + -ATPase activation by inhibiting PP2C.D family protein phosphatases. Here, we extend these findings by demonstrating that SAUR proteins also inhibit tomato PP2C.D family phosphatases and that AtSAUR19 overexpression in tomato ( Solanum lycopersicum ) confers the same suite of phenotypes as previously reported for Arabidopsis. Furthermore, we employ a custom image-based method for measuring hypocotyl segment elongation with high resolution and a method for measuring cell wall mechanical properties, to add mechanistic details to the emerging description of auxin-mediated cell expansion. We find that constitutive expression of GFP-AtSAUR19 bypasses the normal requirement of auxin for elongation growth by increasing the mechanical extensibility of excised hypocotyl segments. In contrast, hypocotyl segments overexpressing a PP2C.D phosphatase are specifically impaired in auxin-mediated elongation. The time courses of auxin-induced SAUR expression and auxin-dependent elongation growth were closely correlated. These findings indicate that induction of SAUR expression is sufficient to elicit auxin-mediated expansion growth by activating PM H + -ATPases to facilitate apoplast acidification and mechanical wall loosening.