Soft X-Ray Imaging of Cellular Carbon and Nitrogen Distributions in Heterocystous Cyanobacteria. Issue 1 (26th March 2018)

• Record Type: Journal Article
• Authors: Teramoto, Takahiro; Azai, Chihiro; Terauchi, Kazuki; Yoshimura, Masashi; Ohta, Toshiaki
• Is Part Of: Plant physiology. Volume 177:Issue 1(2018)
• Page Start: 52
• Page End: 61
• Publication Date: 2018-03-26
• Subjects: Plant physiology -- Periodicals; Botany -- Periodicals; Periodicals; Electronic journals; 571.2
• DOI: 10.1104/pp.17.01767
• Languages: English
• ISSNs: 0032-0889

Abstract

Abstract : Soft x-ray microscopy is a new method developed to determine cellular carbon and nitrogen concentrations in vivo. Abstract: Soft x-ray microscopy (SXM) is a minimally invasive technique for single-cell high-resolution imaging as well as the visualization of intracellular distributions of light elements such as carbon, nitrogen, and oxygen. We used SXM to observe photosynthesis and nitrogen fixation in the filamentous cyanobacterium Anabaena sp. PCC 7120, which can form heterocysts during nitrogen starvation. Statistical and spectroscopic analyses from SXM images around the K-absorption edge of nitrogen revealed a significant difference in the carbon-to-nitrogen (C/N) ratio between vegetative cells and heterocysts. Application of this analysis to soft x-ray images of Anabaena sp. PCC 7120 revealed inhomogenous C/N ratios in the cells. Furthermore, soft x-ray tomography of Anabaena sp. PCC 7120 revealed differing cellular C/N ratios, indicating different carbon and nitrogen distributions between vegetative cells and heterocysts in three dimensions.