In vitro selection of an RNA aptamer yields an interleukin-6/interleukin-6 receptor interaction inhibitor. Issue 5 (2nd January 2021)

• Record Type: Journal Article
• Authors: Ando, Takehiro; Yamamoto, Mizuki; Takamori, Yukio; Tsukamoto, Keita; Fuji, Daisuke; Kawakami, Takashi
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 85:Issue 5(2021)
• Page Start: 1170
• Page End: 1174
• Publication Date: 2021-01-02
• Subjects: interleukin-6 -- interleukin-6 receptor -- SELEX -- aptamer -- in vitro selection; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1093/bbb/zbaa124
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: Interleukin-6 (IL-6) binds to the IL-6 receptor (IL-6R) subunit, related to autoimmune diseases and cytokine storm in COVID-19. In this study, we performed systematic evolution of ligands by exponential enrichment and identified a novel RNA aptamer. This RNA aptamer not only bound to IL-6R with a dissociation constant of 200 n m, but also inhibited the interaction of IL-6R with IL-6.