Photoperiod Affects the Phenotype of Mitochondrial Complex I Mutants. Issue 1 (16th November 2016)

• Record Type: Journal Article
• Authors: Pétriacq, Pierre; de Bont, Linda; Genestout, Lucie; Hao, Jingfang; Laureau, Constance; Florez-Sarasa, Igor; Rzigui, Touhami; Queval, Guillaume; Gilard, Françoise; Mauve, Caroline; Guérard, Florence; Lamothe-Sibold, Marlène; Marion, Jessica; Fresneau, Chantal; Brown, Spencer; Danon, Antoine; Krieger-Liszkay, Anja; Berthomé, Richard; Ribas-Carbo, Miquel; Tcherkez, Guillaume; Cornic, Gabriel; Pineau, Bernard; Gakière, Bertrand; De Paepe, Rosine
• Is Part Of: Plant physiology. Volume 173:Issue 1(2017)
• Page Start: 434
• Page End: 455
• Publication Date: 2016-11-16
• Subjects: Plant physiology -- Periodicals; Botany -- Periodicals; Periodicals; Electronic journals; 571.2
• DOI: 10.1104/pp.16.01484
• Languages: English
• ISSNs: 0032-0889

Abstract

Abstract : Respiratory complex I mutants do not properly acclimate to long-day conditions in Arabidopsis, demonstrating the importance of mitochondria for the photoperiod response. Abstract: Plant mutants for genes encoding subunits of mitochondrial complex I (CI; NADH:ubiquinone oxidoreductase), the first enzyme of the respiratory chain, display various phenotypes depending on growth conditions. Here, we examined the impact of photoperiod, a major environmental factor controlling plant development, on two Arabidopsis ( Arabidopsis thaliana ) CI mutants: a new insertion mutant interrupted in both ndufs8.1 and ndufs8.2 genes encoding the NDUFS8 subunit and the previously characterized ndufs4 CI mutant. In the long day (LD ) condition, both ndufs8.1 and ndufs8.2 single mutants were indistinguishable from Columbia-0 at phenotypic and biochemical levels, whereas the ndufs8.1 ndufs8.2 double mutant was devoid of detectable holo-CI assembly/activity, showed higher alternative oxidase content/activity, and displayed a growth retardation phenotype similar to that of the ndufs4 mutant. Although growth was more affected in ndufs4 than in ndufs8.1 ndufs8.2 under the short day (SD ) condition, both mutants displayed a similar impairment of growth acceleration after transfer to LD compared with the wild type. Untargeted and targeted metabolomics showed that overall metabolism was less responsive to the SD -to-LD transition in mutants than in the wild type. The typical LD acclimation of carbon and nitrogen assimilation as well as redox-related parameters was not observed in ndufs8.1 ndufs8 . Similarly, NAD(H) content, which was higher in the SD condition in both mutants than in Columbia-0, did not adjust under LD . We propose that altered redox homeostasis and NAD(H) content/redox state control the phenotype of CI mutants and photoperiod acclimation in Arabidopsis.