Strain improvement of Lentzea sp. 7887 for higher yield per unit volume on hydroxylation of cyclosporine derivative FR901459. Issue 7 (3rd July 2017)

• Record Type: Journal Article
• Authors: Yabutani, Tetsuya; Tsujimoto, Mami; Ohira, Shunsuke; Shimizu, Shiho; Nakano, Hideo
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 81:Issue 7(2017)
• Page Start: 1456
• Page End: 1459
• Publication Date: 2017-07-03
• Subjects: mutation -- bioconversion -- Lentzea -- pilot scale -- cyclosporine derivative; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2017.1314759
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: A Gram-positive bacterium Lentzea sp. 7887 hydroxylates a cyclosporine derivative FR901459 into AS1837812 (9-hydroxide), which is an important intermediate of candidate drugs that target the hepatitis C virus. We screened a UV-induced mutant, named M-1, which showed about 1.2-fold higher conversion yields, 2-fold higher substrate concentrations (3.69 mM), and 2.5-fold higher yield per unit volume than the wild-type strain.